Abstract
Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1385-1390 |
| Number of pages | 6 |
| Journal | Archives of Gynecology and Obstetrics |
| Volume | 288 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 2013 |
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All Science Journal Classification (ASJC) codes
- Obstetrics and Gynecology
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Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India. / Santhanam, Shanmughapriya; Senthilkumar, G.; Arun, Seshachalam; Vinodhini, Krishnakumar; Sudhakar, Sivasubramanian; Natarajaseenivasan, Kalimuthusamy.
In: Archives of Gynecology and Obstetrics, Vol. 288, No. 6, 01.12.2013, p. 1385-1390.Research output: Contribution to journal › Article
TY - JOUR
T1 - Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India
AU - Santhanam, Shanmughapriya
AU - Senthilkumar, G.
AU - Arun, Seshachalam
AU - Vinodhini, Krishnakumar
AU - Sudhakar, Sivasubramanian
AU - Natarajaseenivasan, Kalimuthusamy
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.
AB - Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.
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U2 - 10.1007/s00404-013-2892-y
DO - 10.1007/s00404-013-2892-y
M3 - Article
VL - 288
SP - 1385
EP - 1390
JO - Archives of Gynecology and Obstetrics
JF - Archives of Gynecology and Obstetrics
SN - 0932-0067
IS - 6
ER -