Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India

Shanmughapriya Santhanam, G. Senthilkumar, Seshachalam Arun, Krishnakumar Vinodhini, Sivasubramanian Sudhakar, Kalimuthusamy Natarajaseenivasan

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Abstract

Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.

Original languageEnglish (US)
Pages (from-to)1385-1390
Number of pages6
JournalArchives of Gynecology and Obstetrics
Volume288
Issue number6
DOIs
StatePublished - Dec 1 2013

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India
Alleles
Carcinoma
Genotype
Valine
Ovarian Neoplasms
Proteins
Proto-Oncogenes
Genetic Predisposition to Disease
Restriction Fragment Length Polymorphisms
Population
Carcinogenesis
Immunohistochemistry
Polymerase Chain Reaction
DNA
Neoplasms

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Santhanam, Shanmughapriya ; Senthilkumar, G. ; Arun, Seshachalam ; Vinodhini, Krishnakumar ; Sudhakar, Sivasubramanian ; Natarajaseenivasan, Kalimuthusamy. / Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India. In: Archives of Gynecology and Obstetrics. 2013 ; Vol. 288, No. 6. pp. 1385-1390.
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abstract = "Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 {\%} of the case patients and 16.3 {\%} of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 {\%}) and 3 control subjects (1.1 {\%}) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 {\%}CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.",
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Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India. / Santhanam, Shanmughapriya; Senthilkumar, G.; Arun, Seshachalam; Vinodhini, Krishnakumar; Sudhakar, Sivasubramanian; Natarajaseenivasan, Kalimuthusamy.

In: Archives of Gynecology and Obstetrics, Vol. 288, No. 6, 01.12.2013, p. 1385-1390.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Polymorphism and overexpression of HER2/neu among ovarian carcinoma women from Tiruchirapalli, Tamil Nadu, India

AU - Santhanam, Shanmughapriya

AU - Senthilkumar, G.

AU - Arun, Seshachalam

AU - Vinodhini, Krishnakumar

AU - Sudhakar, Sivasubramanian

AU - Natarajaseenivasan, Kalimuthusamy

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.

AB - Purpose: Alteration and overexpression of HER2 proto-oncogene have been implicated in the carcinogenesis and prognosis of ovarian cancer. We evaluated this hypothesis among women with ovarian carcinoma patients from Tiruchirapalli, Tamil Nadu, India. Methods: Genomic DNA was extracted from 72 case patients and 288 control subjects and was examined for I655V polymorphism by PCR-RFLP based assay. Immunohistochemistry analysis was carried out in order to study the overexpression of HER2 protein. The observed number of each genotype was compared with that expected for a population in the Hardy-Weinberg equilibrium. In analysing the relation between genotype and overexpression of HER2 protein, Cochran-Mantel-Haenszel statistics was used. Results: We found that 20.8 % of the case patients and 16.3 % of the control subjects were heterozygous for the Val allele and 10 case patients (13.8 %) and 3 control subjects (1.1 %) were homozygous for this allele (P < 0.001). Compared with women with Ile/Ile genotype, women with Val/Val genotype had an elevated risk of ovarian cancer. The genotype distributions were consistent with the Hardy-Weinberg equilibrium. The risk increased with the number of Val allele and women homozygous for the Val allele had 15-fold (OR = 15.3; 95 %CI = 4.09-57.31) increased risk of cancer. The patients homozygous for the Valine allele showed strong HER2 protein expression. Conclusion: The results showed that the valine allele may be an indicator of genetic susceptibility to ovarian carcinoma in the study population.

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U2 - 10.1007/s00404-013-2892-y

DO - 10.1007/s00404-013-2892-y

M3 - Article

VL - 288

SP - 1385

EP - 1390

JO - Archives of Gynecology and Obstetrics

JF - Archives of Gynecology and Obstetrics

SN - 0932-0067

IS - 6

ER -