Polyvalent Display of Biomolecules on Live Cells

Peng Shi; Nan Zhao; Jinping Lai; James Coyne; Erin R. Gaddes; Yong Wang

doi:10.1002/anie.201712596

Polyvalent Display of Biomolecules on Live Cells

Peng Shi, Nan Zhao, Jinping Lai, James Coyne, Erin R. Gaddes, Yong Wang

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Surface display of biomolecules on live cells offers new opportunities to treat human diseases and perform basic studies. Existing methods are primarily focused on monovalent functionalization, that is, the display of single biomolecules across the cell surface. Here we show that the surface of live cells can be functionalized to display polyvalent biomolecular structures through two-step reactions under physiological conditions. This polyvalent functionalization enables the cell surface to recognize the microenvironment one order of magnitude more effectively than with monovalent functionalization. Thus, polyvalent display of biomolecules on live cells holds great potential for various biological and biomedical applications.

Original language	English (US)
Pages (from-to)	6800-6804
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	57
Issue number	23
DOIs	https://doi.org/10.1002/anie.201712596
State	Published - Jun 4 2018

All Science Journal Classification (ASJC) codes

Catalysis
Chemistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/anie.201712596

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title = "Polyvalent Display of Biomolecules on Live Cells",

abstract = "Surface display of biomolecules on live cells offers new opportunities to treat human diseases and perform basic studies. Existing methods are primarily focused on monovalent functionalization, that is, the display of single biomolecules across the cell surface. Here we show that the surface of live cells can be functionalized to display polyvalent biomolecular structures through two-step reactions under physiological conditions. This polyvalent functionalization enables the cell surface to recognize the microenvironment one order of magnitude more effectively than with monovalent functionalization. Thus, polyvalent display of biomolecules on live cells holds great potential for various biological and biomedical applications.",

author = "Peng Shi and Nan Zhao and Jinping Lai and James Coyne and Gaddes, {Erin R.} and Yong Wang",

note = "Funding Information: Research reported here was partially supported by the U.S. National Science Foundation (Grant DMR-1322332) and the National Heart, Lung, and Blood Institute of the National Institutes of Health (Grant R01HL122311). We thank the Husk Institute Microscopy Facility (University Park, PA) for technical support. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

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doi = "10.1002/anie.201712596",

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AU - Zhao, Nan

AU - Lai, Jinping

AU - Coyne, James

AU - Gaddes, Erin R.

AU - Wang, Yong

N1 - Funding Information: Research reported here was partially supported by the U.S. National Science Foundation (Grant DMR-1322332) and the National Heart, Lung, and Blood Institute of the National Institutes of Health (Grant R01HL122311). We thank the Husk Institute Microscopy Facility (University Park, PA) for technical support. Publisher Copyright: © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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Y1 - 2018/6/4

N2 - Surface display of biomolecules on live cells offers new opportunities to treat human diseases and perform basic studies. Existing methods are primarily focused on monovalent functionalization, that is, the display of single biomolecules across the cell surface. Here we show that the surface of live cells can be functionalized to display polyvalent biomolecular structures through two-step reactions under physiological conditions. This polyvalent functionalization enables the cell surface to recognize the microenvironment one order of magnitude more effectively than with monovalent functionalization. Thus, polyvalent display of biomolecules on live cells holds great potential for various biological and biomedical applications.

AB - Surface display of biomolecules on live cells offers new opportunities to treat human diseases and perform basic studies. Existing methods are primarily focused on monovalent functionalization, that is, the display of single biomolecules across the cell surface. Here we show that the surface of live cells can be functionalized to display polyvalent biomolecular structures through two-step reactions under physiological conditions. This polyvalent functionalization enables the cell surface to recognize the microenvironment one order of magnitude more effectively than with monovalent functionalization. Thus, polyvalent display of biomolecules on live cells holds great potential for various biological and biomedical applications.

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Polyvalent Display of Biomolecules on Live Cells

Abstract

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