Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria

Imane Laraba, Houda Boureghda, Nora Abdallah, Oussama Bouaicha, Friday Obanor, Antonio Moretti, David Michael Geiser, Hye Seon Kim, Susan P. McCormick, Robert H. Proctor, Amy C. Kelly, Todd J. Ward, Kerry O'Donnell

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.

Original languageEnglish (US)
Pages (from-to)34-41
Number of pages8
JournalFungal Genetics and Biology
Volume103
DOIs
StatePublished - Jun 1 2017

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Trichothecenes
Algeria
Mycotoxins
Genetic Structures
Fusarium
Population Genetics
Crowns
Triticum
Polymerase Chain Reaction
Genotype
Population
Bayes Theorem
Microsatellite Repeats
Italy
Clone Cells
Alleles
deoxynivalenol

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

Laraba, Imane ; Boureghda, Houda ; Abdallah, Nora ; Bouaicha, Oussama ; Obanor, Friday ; Moretti, Antonio ; Geiser, David Michael ; Kim, Hye Seon ; McCormick, Susan P. ; Proctor, Robert H. ; Kelly, Amy C. ; Ward, Todd J. ; O'Donnell, Kerry. / Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria. In: Fungal Genetics and Biology. 2017 ; Vol. 103. pp. 34-41.
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abstract = "Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8{\%} 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77{\%}) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89{\%} of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.",
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Laraba, I, Boureghda, H, Abdallah, N, Bouaicha, O, Obanor, F, Moretti, A, Geiser, DM, Kim, HS, McCormick, SP, Proctor, RH, Kelly, AC, Ward, TJ & O'Donnell, K 2017, 'Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria', Fungal Genetics and Biology, vol. 103, pp. 34-41. https://doi.org/10.1016/j.fgb.2017.04.001

Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria. / Laraba, Imane; Boureghda, Houda; Abdallah, Nora; Bouaicha, Oussama; Obanor, Friday; Moretti, Antonio; Geiser, David Michael; Kim, Hye Seon; McCormick, Susan P.; Proctor, Robert H.; Kelly, Amy C.; Ward, Todd J.; O'Donnell, Kerry.

In: Fungal Genetics and Biology, Vol. 103, 01.06.2017, p. 34-41.

Research output: Contribution to journalArticle

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T1 - Population genetic structure and mycotoxin potential of the wheat crown rot and head blight pathogen Fusarium culmorum in Algeria

AU - Laraba, Imane

AU - Boureghda, Houda

AU - Abdallah, Nora

AU - Bouaicha, Oussama

AU - Obanor, Friday

AU - Moretti, Antonio

AU - Geiser, David Michael

AU - Kim, Hye Seon

AU - McCormick, Susan P.

AU - Proctor, Robert H.

AU - Kelly, Amy C.

AU - Ward, Todd J.

AU - O'Donnell, Kerry

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.

AB - Surveys for crown rot (FCR) and head blight (FHB) of Algerian wheat conducted during 2014 and 2015 revealed that Fusarium culmorum strains producing 3-acetyl-deoxynivalenol (3ADON) or nivalenol (NIV) were the causal agents of these important diseases. Morphological identification of the isolates (n FCR = 110, n FHB = 30) was confirmed by sequencing a portion of TEF1. To assess mating type idiomorph, trichothecene chemotype potential and global population structure, the Algerian strains were compared with preliminary sample of F. culmorum from Italy (n = 27), Australia (n = 30) and the United States (n = 28). A PCR assay for MAT idiomorph revealed that MAT1-1 and MAT1-2 strains were segregating in nearly equal proportions, except within Algeria where two-thirds of the strains were MAT1-2. An allele-specific PCR assay indicated that the 3ADON trichothecene genotype was predominant globally (83.8% 3ADON) and in each of the four countries sampled. In vitro toxin analyses confirmed trichothecene genotype PCR data and demonstrated that most of the strains tested (77%) produced culmorin. Global population genetic structure of 191 strains was assessed using nine microsatellite markers (SSRs). AMOVA of the clone corrected data indicated that 89% of the variation was within populations. Bayesian analysis of the SSR data identified two globally distributed, sympatric populations within which both trichothecene chemotypes and mating types were represented.

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