Populus euphratica: The transcriptomic response to drought stress

Sha Tang, Haiying Liang, Donghui Yan, Ying Zhao, Xiao Han, John Edward Carlson, Xinli Xia, Weilun Yin

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Populus euphratica Olivier is widely established in arid and semiarid regions but lags in the availability of transcriptomic resources in response to water deficiency. To investigate the mechanisms that allow P. euphratica to maintain growth in arid regions, the responses of the plant to soil water deficit were analyzed at a systems level using physiological and pyrosequencing approaches. We generated 218,601 and 287,120 reads from non-stressed control and drought-stressed P. euphratica leaves respectively, totaling over 200 million base pairs. After assembly, 24,013 transcripts were yielded with an average length of 1,128 bp. We determined 2,279 simple sequence repeats, which may have possible information for understanding drought adaption of woody plants. Stomatal closure was inhibited under moderate drought to maintain a relatively high rate of CO2 assimilation and water transportation, which was supposed to be important for P. euphratica to maintain normal growth and develop vigorous root systems in an adverse environment. This was accompanied by strong transcriptional remodeling of stress-perception, signaling and transcription regulation, photoprotective system, oxidative stress detoxification, and other stress responsive genes. In addition, genes involved in stomatal closure inhibition, ascorbate-glutathione pathway and ubiquitin-proteasome system that may specially modulate the drought stress responses of P. euphratica are highlighted. Our analysis provides a comprehensive picture of how P. euphratica responds to drought stress at physiological and transcriptome levels which may help to understand molecular mechanisms associated with drought response and could be useful for genetic engineering of woody plants.

Original languageEnglish (US)
Pages (from-to)539-557
Number of pages19
JournalPlant molecular biology
Volume83
Issue number6
DOIs
StatePublished - Dec 1 2013

Fingerprint

Populus euphratica
Populus
Droughts
transcriptomics
water stress
drought
woody plants
Water
water transportation
soil water deficit
Genetic Engineering
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
ubiquitin
Growth
Ubiquitin
genetic engineering
Transcriptome
transcriptome
Base Pairing

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Tang, Sha ; Liang, Haiying ; Yan, Donghui ; Zhao, Ying ; Han, Xiao ; Carlson, John Edward ; Xia, Xinli ; Yin, Weilun. / Populus euphratica : The transcriptomic response to drought stress. In: Plant molecular biology. 2013 ; Vol. 83, No. 6. pp. 539-557.
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abstract = "Populus euphratica Olivier is widely established in arid and semiarid regions but lags in the availability of transcriptomic resources in response to water deficiency. To investigate the mechanisms that allow P. euphratica to maintain growth in arid regions, the responses of the plant to soil water deficit were analyzed at a systems level using physiological and pyrosequencing approaches. We generated 218,601 and 287,120 reads from non-stressed control and drought-stressed P. euphratica leaves respectively, totaling over 200 million base pairs. After assembly, 24,013 transcripts were yielded with an average length of 1,128 bp. We determined 2,279 simple sequence repeats, which may have possible information for understanding drought adaption of woody plants. Stomatal closure was inhibited under moderate drought to maintain a relatively high rate of CO2 assimilation and water transportation, which was supposed to be important for P. euphratica to maintain normal growth and develop vigorous root systems in an adverse environment. This was accompanied by strong transcriptional remodeling of stress-perception, signaling and transcription regulation, photoprotective system, oxidative stress detoxification, and other stress responsive genes. In addition, genes involved in stomatal closure inhibition, ascorbate-glutathione pathway and ubiquitin-proteasome system that may specially modulate the drought stress responses of P. euphratica are highlighted. Our analysis provides a comprehensive picture of how P. euphratica responds to drought stress at physiological and transcriptome levels which may help to understand molecular mechanisms associated with drought response and could be useful for genetic engineering of woody plants.",
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Tang, S, Liang, H, Yan, D, Zhao, Y, Han, X, Carlson, JE, Xia, X & Yin, W 2013, 'Populus euphratica: The transcriptomic response to drought stress', Plant molecular biology, vol. 83, no. 6, pp. 539-557. https://doi.org/10.1007/s11103-013-0107-3

Populus euphratica : The transcriptomic response to drought stress. / Tang, Sha; Liang, Haiying; Yan, Donghui; Zhao, Ying; Han, Xiao; Carlson, John Edward; Xia, Xinli; Yin, Weilun.

In: Plant molecular biology, Vol. 83, No. 6, 01.12.2013, p. 539-557.

Research output: Contribution to journalArticle

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T2 - The transcriptomic response to drought stress

AU - Tang, Sha

AU - Liang, Haiying

AU - Yan, Donghui

AU - Zhao, Ying

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AU - Carlson, John Edward

AU - Xia, Xinli

AU - Yin, Weilun

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