Positionally independent and exchangeable late budding functions of the rous sarcoma virus and human immunodeficiency virus Gag proteins

L. J. Parent, R. P. Bennett, R. C. Craven, T. D. Nelle, N. K. Krishna, J. B. Bowzard, C. B. Wilson, B. A. Puffer, R. C. Montelaro, J. W. Wills

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

The Gag proteins of Rous sarcoma virus and human immunodeficiency virus (HIV) each contain a function involved in a late step in budding, defects in which result in the accumulation of these molecules at the plasma membrane. In the Rous sarcoma virus Gag protein (Pr76(gag)), this assembly domain is associated with a PPPY motif, which is located at an internal position between the MA and CA sequences. This motif is not contained anywhere within the HIV Gag protein (Pr55(gag)), and the MA sequence is linked directly to CA. Instead, a late assembly function of HIV has been associated with the p6 sequence situated at the C terminus of Gag. Here we demonstrate the remarkable finding that the late assembly domains from these two unrelated Gag proteins are exchangeable between retroviruses and can function in a positionally independent manner.

Original languageEnglish (US)
Pages (from-to)5455-5460
Number of pages6
JournalJournal of Virology
Volume69
Issue number9
StatePublished - 1995

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Human Immunodeficiency Virus gag Gene Products
Rous sarcoma virus
gag Gene Products
Human immunodeficiency virus
HIV
Retroviridae
proteins
Cell Membrane
plasma membrane

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Parent, L. J. ; Bennett, R. P. ; Craven, R. C. ; Nelle, T. D. ; Krishna, N. K. ; Bowzard, J. B. ; Wilson, C. B. ; Puffer, B. A. ; Montelaro, R. C. ; Wills, J. W. / Positionally independent and exchangeable late budding functions of the rous sarcoma virus and human immunodeficiency virus Gag proteins. In: Journal of Virology. 1995 ; Vol. 69, No. 9. pp. 5455-5460.
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Parent, LJ, Bennett, RP, Craven, RC, Nelle, TD, Krishna, NK, Bowzard, JB, Wilson, CB, Puffer, BA, Montelaro, RC & Wills, JW 1995, 'Positionally independent and exchangeable late budding functions of the rous sarcoma virus and human immunodeficiency virus Gag proteins', Journal of Virology, vol. 69, no. 9, pp. 5455-5460.

Positionally independent and exchangeable late budding functions of the rous sarcoma virus and human immunodeficiency virus Gag proteins. / Parent, L. J.; Bennett, R. P.; Craven, R. C.; Nelle, T. D.; Krishna, N. K.; Bowzard, J. B.; Wilson, C. B.; Puffer, B. A.; Montelaro, R. C.; Wills, J. W.

In: Journal of Virology, Vol. 69, No. 9, 1995, p. 5455-5460.

Research output: Contribution to journalArticle

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AU - Parent, L. J.

AU - Bennett, R. P.

AU - Craven, R. C.

AU - Nelle, T. D.

AU - Krishna, N. K.

AU - Bowzard, J. B.

AU - Wilson, C. B.

AU - Puffer, B. A.

AU - Montelaro, R. C.

AU - Wills, J. W.

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