Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors

Katsuhiko Murakami, Jeffrey T. Owens, Tamara A. Belyaeva, Claude F. Meares, Stephen J.W. Busby, Akira Ishihama

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Interactions between the cAMP receptor protein (CRP) and the carboxy- terminal regulatory domain (CTD) of Escherichia coli RNA polymerase α subunit were analyzed at promoters carrying tandem DNA sites for CRP binding using a chemical nuclease covalently attached to α each CRP dimer was found to direct the positioning of one of the two α subunit CTDs. Thus, the function of RNA polymerase may be subject to regulation through protein- protein interactions between the two α subunits and two different species of transcription factors.

Original languageEnglish (US)
Pages (from-to)11274-11278
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number21
DOIs
StatePublished - Oct 14 1997

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Cyclic AMP Receptor Protein
DNA-Directed RNA Polymerases
Transcription Factors
Protein Binding
Proteins
Escherichia coli
DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

Murakami, Katsuhiko ; Owens, Jeffrey T. ; Belyaeva, Tamara A. ; Meares, Claude F. ; Busby, Stephen J.W. ; Ishihama, Akira. / Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 21. pp. 11274-11278.
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Positioning of two alpha subunit carboxy-terminal domains of RNA polymerase at promoters by two transcription factors. / Murakami, Katsuhiko; Owens, Jeffrey T.; Belyaeva, Tamara A.; Meares, Claude F.; Busby, Stephen J.W.; Ishihama, Akira.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 21, 14.10.1997, p. 11274-11278.

Research output: Contribution to journalArticle

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AU - Owens, Jeffrey T.

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AU - Busby, Stephen J.W.

AU - Ishihama, Akira

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