Possible antioxidant action of the Bcl-2 protein

K. Marshall, L. Ellerby, Hong-Gang Wang, J. C. Reed, D. E. Bredesen, O. I. Aruoma, B. Halliwell

Research output: Contribution to journalArticle

Abstract

Reactive oxygen species (ROS) have been implicated as mediators of programmed cell death (PCD). PCD is involved in many physiological and pathological processes. The Bcl-2 protein suppresses various types of apoptotic and in some cases necrotic cell death. The Bcl-2 protein is located at sites of free radical generation such as mitochondria and endoplasmic reticulum. However, it's mechanism of action is unknown. It has been suggested the Bcl-2 protein may act via an antioxidant mechanism to prevent PCD. To determine whether the Bcl-2 protein itself has antioxidant properties we tested the purified protein directly in our assay systems. We compared the action of the Bcl-2 protein with that of well known antioxidant proteins: catalase, glutathione peroxidase and Superoxide dismutase. We conclude that, in our assay systems, Bcl-2 appears not to show any significant 02'", H2U2 or organic peroxide-removing activity when compared with the above proteins.

Original languageEnglish (US)
JournalBiochemical Society Transactions
Volume24
Issue number4
StatePublished - Jan 1 1996

Fingerprint

Antioxidants
Cell death
Proteins
Cell Death
Assays
Physiological Phenomena
Mitochondria
Peroxides
Pathologic Processes
Glutathione Peroxidase
Endoplasmic Reticulum
Catalase
Superoxide Dismutase
Free Radicals
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Marshall, K., Ellerby, L., Wang, H-G., Reed, J. C., Bredesen, D. E., Aruoma, O. I., & Halliwell, B. (1996). Possible antioxidant action of the Bcl-2 protein. Biochemical Society Transactions, 24(4).
Marshall, K. ; Ellerby, L. ; Wang, Hong-Gang ; Reed, J. C. ; Bredesen, D. E. ; Aruoma, O. I. ; Halliwell, B. / Possible antioxidant action of the Bcl-2 protein. In: Biochemical Society Transactions. 1996 ; Vol. 24, No. 4.
@article{eb2b70c9a7b64c9aacb583828be2208b,
title = "Possible antioxidant action of the Bcl-2 protein",
abstract = "Reactive oxygen species (ROS) have been implicated as mediators of programmed cell death (PCD). PCD is involved in many physiological and pathological processes. The Bcl-2 protein suppresses various types of apoptotic and in some cases necrotic cell death. The Bcl-2 protein is located at sites of free radical generation such as mitochondria and endoplasmic reticulum. However, it's mechanism of action is unknown. It has been suggested the Bcl-2 protein may act via an antioxidant mechanism to prevent PCD. To determine whether the Bcl-2 protein itself has antioxidant properties we tested the purified protein directly in our assay systems. We compared the action of the Bcl-2 protein with that of well known antioxidant proteins: catalase, glutathione peroxidase and Superoxide dismutase. We conclude that, in our assay systems, Bcl-2 appears not to show any significant 02'{"}, H2U2 or organic peroxide-removing activity when compared with the above proteins.",
author = "K. Marshall and L. Ellerby and Hong-Gang Wang and Reed, {J. C.} and Bredesen, {D. E.} and Aruoma, {O. I.} and B. Halliwell",
year = "1996",
month = "1",
day = "1",
language = "English (US)",
volume = "24",
journal = "Biochemical Society Transactions",
issn = "0300-5127",
publisher = "Portland Press Ltd.",
number = "4",

}

Marshall, K, Ellerby, L, Wang, H-G, Reed, JC, Bredesen, DE, Aruoma, OI & Halliwell, B 1996, 'Possible antioxidant action of the Bcl-2 protein', Biochemical Society Transactions, vol. 24, no. 4.

Possible antioxidant action of the Bcl-2 protein. / Marshall, K.; Ellerby, L.; Wang, Hong-Gang; Reed, J. C.; Bredesen, D. E.; Aruoma, O. I.; Halliwell, B.

In: Biochemical Society Transactions, Vol. 24, No. 4, 01.01.1996.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Possible antioxidant action of the Bcl-2 protein

AU - Marshall, K.

AU - Ellerby, L.

AU - Wang, Hong-Gang

AU - Reed, J. C.

AU - Bredesen, D. E.

AU - Aruoma, O. I.

AU - Halliwell, B.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Reactive oxygen species (ROS) have been implicated as mediators of programmed cell death (PCD). PCD is involved in many physiological and pathological processes. The Bcl-2 protein suppresses various types of apoptotic and in some cases necrotic cell death. The Bcl-2 protein is located at sites of free radical generation such as mitochondria and endoplasmic reticulum. However, it's mechanism of action is unknown. It has been suggested the Bcl-2 protein may act via an antioxidant mechanism to prevent PCD. To determine whether the Bcl-2 protein itself has antioxidant properties we tested the purified protein directly in our assay systems. We compared the action of the Bcl-2 protein with that of well known antioxidant proteins: catalase, glutathione peroxidase and Superoxide dismutase. We conclude that, in our assay systems, Bcl-2 appears not to show any significant 02'", H2U2 or organic peroxide-removing activity when compared with the above proteins.

AB - Reactive oxygen species (ROS) have been implicated as mediators of programmed cell death (PCD). PCD is involved in many physiological and pathological processes. The Bcl-2 protein suppresses various types of apoptotic and in some cases necrotic cell death. The Bcl-2 protein is located at sites of free radical generation such as mitochondria and endoplasmic reticulum. However, it's mechanism of action is unknown. It has been suggested the Bcl-2 protein may act via an antioxidant mechanism to prevent PCD. To determine whether the Bcl-2 protein itself has antioxidant properties we tested the purified protein directly in our assay systems. We compared the action of the Bcl-2 protein with that of well known antioxidant proteins: catalase, glutathione peroxidase and Superoxide dismutase. We conclude that, in our assay systems, Bcl-2 appears not to show any significant 02'", H2U2 or organic peroxide-removing activity when compared with the above proteins.

UR - http://www.scopus.com/inward/record.url?scp=33749187491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749187491&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749187491

VL - 24

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - 4

ER -

Marshall K, Ellerby L, Wang H-G, Reed JC, Bredesen DE, Aruoma OI et al. Possible antioxidant action of the Bcl-2 protein. Biochemical Society Transactions. 1996 Jan 1;24(4).