Possible mechanism for cold denaturation of proteins at high pressure

Manuel I. Marqués, Jose M. Borreguero, H. Eugene Stanley, Nikolay Dokholyan

Research output: Contribution to journalArticle

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Abstract

We study cold denaturation of proteins at high pressures. Using multicanonical Monte Carlo simulations of a model protein in a water bath, we investigate the effect of water density fluctuations on protein stability. We find that above the pressure where water freezes to the dense ice phase ([Formula presented]) the mechanism for cold denaturation with decreasing temperature is the loss of local low-density water structure. We find our results in agreement with data of bovine pancreatic ribonuclease [Formula presented].

Original languageEnglish (US)
JournalPhysical Review Letters
Volume91
Issue number13
DOIs
StatePublished - Jan 1 2003

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biopolymer denaturation
proteins
water
water pressure
baths
ice
simulation
temperature

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Marqués, Manuel I. ; Borreguero, Jose M. ; Stanley, H. Eugene ; Dokholyan, Nikolay. / Possible mechanism for cold denaturation of proteins at high pressure. In: Physical Review Letters. 2003 ; Vol. 91, No. 13.
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Possible mechanism for cold denaturation of proteins at high pressure. / Marqués, Manuel I.; Borreguero, Jose M.; Stanley, H. Eugene; Dokholyan, Nikolay.

In: Physical Review Letters, Vol. 91, No. 13, 01.01.2003.

Research output: Contribution to journalArticle

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