Post-column derivatization for capillary electrophoresis using naphthalene-2,3-dicarboxaldehyde and 2-mercaptoethanol

S. Douglass Gilman, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

A reagent consisting of naphthalene-2,3-dicarboxaldehyde and 2-mercaptoethanol has been investigated for post-column derivatization of amines with a gap-based reactor developed for capillary electrophoresis and laser-induced fluorescence detection. Separation and detection of a wide range of compounds has been demonstrated including neuropeptides, catecholamines, amino acids and proteins. Compounds derivatized with this reagent have been detected by laser-induced fluorescence using excitation with the He-Cd laser (442 nm line) and could also be detected using an Ar ion laser (458 nm line). Mass detection limits obtained with this reagent and post-column derivatization for glycine and transferrin are 330 and 13 mol, respectively. Sensitive detection of lysine has also been demonstrated with this reagent. Electropherograms for homogenate samples of a snail brain and for single mammalian cells have been obtained using post-column derivatization with this reagent and laser-induced fluorescence detection.

Original languageEnglish (US)
Pages (from-to)133-141
Number of pages9
JournalAnalytical methods & instrumentation
Volume2
Issue number3
StatePublished - 1995

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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