Post-transcriptional regulation on a global scale: Form and function of Csr/Rsm systems

Tony Romeo, Christopher A. Vakulskas, Paul Lee Babitzke

Research output: Contribution to journalShort survey

182 Citations (Scopus)

Abstract

Originally described as a repressor of gene expression in the stationary phase of growth, CsrA (RsmA) regulates primary and secondary metabolic pathways, biofilm formation, motility, virulence circuitry of pathogens, quorum sensing and stress response systems by binding to conserved sequences in its target mRNAs and altering their translation and/or turnover. While the binding of CsrA to RNA is understood at an atomic level, new mechanisms of gene activation and repression by this protein are still emerging. In the γ-proteobacteria, small non-coding RNAs (sRNAs) use molecular mimicry to sequester multiple CsrA dimers away from mRNA. In contrast, the FliW protein of Bacillus subtilis inhibits CsrA activity by binding to this protein, thereby establishing a checkpoint in flagellum morphogenesis. Turnover of CsrB and CsrC sRNAs in Escherichia coli requires a specificity protein of the GGDEF-EAL domain superfamily, CsrD, in addition to the housekeeping nucleases RNase E and PNPase. The Csr system of E.coli contains extensive autoregulatory circuitry, which governs the expression and activity of CsrA. Interaction of the Csr system with transcriptional regulatory networks results in a variety of complex response patterns. This minireview will highlight basic principles and new insights into the workings of these complex eubacterial regulatory systems.

Original languageEnglish (US)
Pages (from-to)313-324
Number of pages12
JournalEnvironmental microbiology
Volume15
Issue number2
DOIs
StatePublished - Feb 1 2013

Fingerprint

Small Untranslated RNA
RNA
protein
Escherichia coli
Molecular Mimicry
Proteobacteria
Quorum Sensing
Housekeeping
Messenger RNA
turnover
Proteins
Flagella
proteins
Conserved Sequence
Gene Regulatory Networks
molecular mimicry
Biofilms
Metabolic Networks and Pathways
Bacillus subtilis
Morphogenesis

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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title = "Post-transcriptional regulation on a global scale: Form and function of Csr/Rsm systems",
abstract = "Originally described as a repressor of gene expression in the stationary phase of growth, CsrA (RsmA) regulates primary and secondary metabolic pathways, biofilm formation, motility, virulence circuitry of pathogens, quorum sensing and stress response systems by binding to conserved sequences in its target mRNAs and altering their translation and/or turnover. While the binding of CsrA to RNA is understood at an atomic level, new mechanisms of gene activation and repression by this protein are still emerging. In the γ-proteobacteria, small non-coding RNAs (sRNAs) use molecular mimicry to sequester multiple CsrA dimers away from mRNA. In contrast, the FliW protein of Bacillus subtilis inhibits CsrA activity by binding to this protein, thereby establishing a checkpoint in flagellum morphogenesis. Turnover of CsrB and CsrC sRNAs in Escherichia coli requires a specificity protein of the GGDEF-EAL domain superfamily, CsrD, in addition to the housekeeping nucleases RNase E and PNPase. The Csr system of E.coli contains extensive autoregulatory circuitry, which governs the expression and activity of CsrA. Interaction of the Csr system with transcriptional regulatory networks results in a variety of complex response patterns. This minireview will highlight basic principles and new insights into the workings of these complex eubacterial regulatory systems.",
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Post-transcriptional regulation on a global scale : Form and function of Csr/Rsm systems. / Romeo, Tony; Vakulskas, Christopher A.; Babitzke, Paul Lee.

In: Environmental microbiology, Vol. 15, No. 2, 01.02.2013, p. 313-324.

Research output: Contribution to journalShort survey

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