Postattachment neutralization of papillomaviruses by monoclonal and polyclonal antibodies

Neil Christensen, Nancy M. Cladel, Cynthia A. Reed

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Postattachment neutralization of papillomaviruses (PVs) was analyzed in three PV-infectivity models: (i) the BPV-1-induced focus-forming assay using C127 cells; (ii) in vitro abortive infection of rabbit RK-13 and Sf1Ep cells with CRPV; and (iii) HPV-11-induced morphological transformation of human foreskin chips in the athymic mouse xenograft system. In each assay system, aliquots of infectious virus were added to the appropriate target cells and incubated at 37°, followed at various postinfection time intervals with neutralizing monoclonal antibodies (N-MAbs) that target surface conformational epitopes. In all three model systems, the N-MAbs were able to neutralize PV infection when added as late as 8 hr after addition of infectious PV to host cells. These results imply that papillomaviruses attach to but do not penetrate inside host cells for a significant period of time and that the bound virus is thus still susceptible to neutralization by neutralizing antibodies.

Original languageEnglish (US)
Article number71059
Pages (from-to)136-142
Number of pages7
JournalVirology
Volume207
Issue number1
DOIs
StatePublished - Feb 20 1995

All Science Journal Classification (ASJC) codes

  • Virology

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