Postnatal maturation of gephyrin/glycine receptor cluster on developing Renshaw cells

Kevin D. Alloway, Joshua J. Mutic, Zachary S. Hoffer, John E. Hoover

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Adult mammalian Renshaw cells express large and complex postsynaptic gephyrin/glycine receptor clusters on their surface. Larger gephyrin clusters correlate with more 'efficacious' inhibitory synapses, in terms of larger postsynaptic quantal size amplitudes, in part because they likely contain more postsynaptic receptors (Lim et al. [1999] J. Physiol. (Lond.) 516:505-512; Oleskevich et al. [1999] J. Neurophysiology 82:312-319). Here, we studied the postnatal development of the gephyrin/glycine receptor cluster size on Renshaw cells. Renshaw cells were identified by their calbindin immunoreactivity, location and morphology, and presence of cholinergic input. The populations of clusters over developing Renshaw cells immunoreactive to gephyrin or glycine receptor α1 subunits were comparable in number, size, and complexity and displayed a high degree of colocalization (>90%) at all ages. Quantitative morphologic analysis was performed on gephyrin-immunoreactive clusters. In neonatal animals, Renshaw cells expressed small punctate gephyrin-immunoreactive clusters (mean cluster size ± SD = 0.19 ± 0.19 μm2 at 2 days; 0.22 ± 0.19 μm2 at 5 days). By 10 and 15 days of age, Renshaw cells exhibited gephyrin-immunoreactive clusters that were larger and more complex (0.32 ± 0.19 μm2 at 10 days; 0.41 ± 0.32 μm2 at 15 days). Cluster growth reached a plateau in 25- and 60-day-old Renshaw cells (0.45 ± 0.43 μm2; 0.56 ± 0.55 μm2, respectively). By using electron microscopy, we confirmed that gephyrin-immunoreactive clusters were located at postsynaptic sites at both early and late postnatal ages on Renshaw cells. The potential significance of this gephyrin/glycine receptor cluster size maturation that sets Renshaw cells apart from other interneurons is discussed. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)130-142
Number of pages13
JournalJournal of Comparative Neurology
Volume426
Issue number1
DOIs
StatePublished - Oct 9 2000

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Glycine Receptors
Newborn Animals
gephyrin
Renshaw Cells
Calbindins
Neurophysiology
Interneurons
Synapses
Cholinergic Agents
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Alloway, Kevin D. ; Mutic, Joshua J. ; Hoffer, Zachary S. ; Hoover, John E. / Postnatal maturation of gephyrin/glycine receptor cluster on developing Renshaw cells. In: Journal of Comparative Neurology. 2000 ; Vol. 426, No. 1. pp. 130-142.
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Postnatal maturation of gephyrin/glycine receptor cluster on developing Renshaw cells. / Alloway, Kevin D.; Mutic, Joshua J.; Hoffer, Zachary S.; Hoover, John E.

In: Journal of Comparative Neurology, Vol. 426, No. 1, 09.10.2000, p. 130-142.

Research output: Contribution to journalArticle

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