Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition

Sujit Pujhari, Tayyba T. Baig, Alexander N. Zakhartchouk

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a serious threat to the pork industry, and its pathogenesis needs further investigations. To study the role of two structural proteins of PRRSV in virus-host cells interactions, two stable cell lines (MARC-2a and MARC-N) expressing GP2 and N proteins, respectively, were established. We induced apoptosis in these cells by treating them with staurosporine and found a significant reduction in the number of apoptotic cells in MARC-2a as compared to MARC-N and MARC-145 cells. In addition, we found significantly higher activities of transcriptional factors (NF-B and AP-1) in both cell lines as compared to MARC-145 (parent cells). Overall, our data suggest that, although both stable cell lines activate NF-B and AP-1, GP2 triggers the antiapoptotic process through an intermediate step that needs to be further investigated.

Original languageEnglish (US)
Article number160505
JournalBioMed Research International
Volume2014
DOIs
StatePublished - Feb 5 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Potential role of porcine reproductive and respiratory syndrome virus structural protein GP2 in apoptosis inhibition'. Together they form a unique fingerprint.

  • Cite this