Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures

Valerie J. Watson, Bruce E. Logan

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480% more power than MR-1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9mWm-2 in the cubic MFC, while MR-1 produced 148 ± 20mWm-2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR-1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately -175mV for mixed and approximately -210mV for MR-1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (∼250mV formixed and ∼300mV for MR-1). Mixed cultures produced more power than MR-1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure andmixed cultures depend on the MFC architecture.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalBiotechnology and bioengineering
Volume105
Issue number3
DOIs
StatePublished - Feb 15 2010

Fingerprint

Shewanella
Oxidation-Reduction
Acoustic impedance
Bottles
Waste Water
Electric Impedance
Anodes
Electrodes
Wastewater
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

@article{65136e038de94bf78f34ef9caaf557be,
title = "Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures",
abstract = "Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480{\%} more power than MR-1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9mWm-2 in the cubic MFC, while MR-1 produced 148 ± 20mWm-2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR-1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately -175mV for mixed and approximately -210mV for MR-1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (∼250mV formixed and ∼300mV for MR-1). Mixed cultures produced more power than MR-1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure andmixed cultures depend on the MFC architecture.",
author = "Watson, {Valerie J.} and Logan, {Bruce E.}",
year = "2010",
month = "2",
day = "15",
doi = "10.1002/bit.22556",
language = "English (US)",
volume = "105",
pages = "489--498",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "3",

}

Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures. / Watson, Valerie J.; Logan, Bruce E.

In: Biotechnology and bioengineering, Vol. 105, No. 3, 15.02.2010, p. 489-498.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures

AU - Watson, Valerie J.

AU - Logan, Bruce E.

PY - 2010/2/15

Y1 - 2010/2/15

N2 - Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480% more power than MR-1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9mWm-2 in the cubic MFC, while MR-1 produced 148 ± 20mWm-2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR-1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately -175mV for mixed and approximately -210mV for MR-1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (∼250mV formixed and ∼300mV for MR-1). Mixed cultures produced more power than MR-1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure andmixed cultures depend on the MFC architecture.

AB - Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480% more power than MR-1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9mWm-2 in the cubic MFC, while MR-1 produced 148 ± 20mWm-2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR-1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately -175mV for mixed and approximately -210mV for MR-1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (∼250mV formixed and ∼300mV for MR-1). Mixed cultures produced more power than MR-1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure andmixed cultures depend on the MFC architecture.

UR - http://www.scopus.com/inward/record.url?scp=74849126212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74849126212&partnerID=8YFLogxK

U2 - 10.1002/bit.22556

DO - 10.1002/bit.22556

M3 - Article

C2 - 19787640

AN - SCOPUS:74849126212

VL - 105

SP - 489

EP - 498

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 3

ER -