Power production in MFCs inoculated with Shewanella oneidensis MR-1 or mixed cultures

Valerie J. Watson, Bruce E. Logan

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

Power densities and oxidation-reduction potentials (ORPs) of MFCs containing a pure culture of Shewanella oneidensis MR-1 were compared to mixed cultures (wastewater inoculum) in cube shaped, 1-, 2-, and 3-bottle batch-fed MFC reactor configurations. The reactor architecture influenced the relative power produced by the different inocula, with the mixed culture generating 68-480% more power than MR-1 in each MFC configuration. The mixed culture produced the maximum power density of 858 ± 9mWm-2 in the cubic MFC, while MR-1 produced 148 ± 20mWm-2. The higher power by the mixed culture was primarily a result of lower internal resistances than those produced by the pure culture. Power was a direct function of ohmic resistance for the mixed culture, but not for strain MR-1. ORP of the anode compartment varied with reactor configuration and inoculum, and it was always negative during maximum power production but it did not vary in proportion to power output. The ORP varied primarily at the end of the cycle when substrate was depleted, with a change from a reductive environment during maximum power production (approximately -175mV for mixed and approximately -210mV for MR-1 in cubic MFCs), to an oxidative environment at the end of the batch cycle (∼250mV formixed and ∼300mV for MR-1). Mixed cultures produced more power than MR-1 MFCs even though their redox potential was less negative. These results demonstrate that differences between power densities produced by pure andmixed cultures depend on the MFC architecture.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalBiotechnology and bioengineering
Volume105
Issue number3
DOIs
StatePublished - Feb 15 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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