PPS nanoparticles as versatile delivery system to induce systemic and broad mucosal immunity after intranasal administration

Armando Stano, André J. van der Vlies, Mikael M. Martino, Melody A. Swartz, Jeffrey A. Hubbell, Eleonora Simeoni

Research output: Contribution to journalArticlepeer-review

Abstract

Degradable polymer nanoparticles (NPs, 50. nm) based on polypropylene sulfide (PPS) were conjugated to thiolated antigen and adjuvant proteins by reversible disulfide bonds and evaluated in mucosal vaccination. Ovalbumin was used as a model antigen, and antigen-conjugated NPs were administered intranasally in the mouse. We show penetration of nasal mucosae, transit via M cells, and uptake by antigen-presenting cells in the nasal-associated lymphoid tissue. Ovalbumin-conjugated NPs induced cytotoxic T lymphocytic responses in lung and spleen tissues, as well as humoral response in mucosal airways. Co-conjugation of the TLR5 ligand flagellin further enhanced humoral responses in the airways as well as in the distant vaginal and rectal mucosal compartments and induced cellular immune responses with a Th1 bias, in contrast with free flagellin. The PPS NP platform thus appears interesting as a platform for intranasally-administered mucosal vaccination for inducing broad mucosal immunity.

Original languageEnglish (US)
Pages (from-to)804-812
Number of pages9
JournalVaccine
Volume29
Issue number4
DOIs
StatePublished - Jan 17 2011

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'PPS nanoparticles as versatile delivery system to induce systemic and broad mucosal immunity after intranasal administration'. Together they form a unique fingerprint.

Cite this