Practical application of a chromogenic FXIIa assay

Rui Zhuo, Erwin A. Vogler

Research output: Contribution to journalArticle

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Abstract

Autohydrolysis of blood factor XII (FXII + FXIIa → 2 FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.

Original languageEnglish (US)
Pages (from-to)4840-4845
Number of pages6
JournalBiomaterials
Volume27
Issue number28
DOIs
StatePublished - Oct 1 2006

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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