Practical application of a chromogenic FXIIa assay

Rui Zhuo; Erwin A. Vogler

doi:10.1016/j.biomaterials.2006.05.008

Practical application of a chromogenic FXIIa assay

Rui Zhuo, Erwin A. Vogler

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Autohydrolysis of blood factor XII (FXII + FXIIa → 2 FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.

Original language	English (US)
Pages (from-to)	4840-4845
Number of pages	6
Journal	Biomaterials
Volume	27
Issue number	28
DOIs	https://doi.org/10.1016/j.biomaterials.2006.05.008
State	Published - Oct 2006

All Science Journal Classification (ASJC) codes

Biophysics
Bioengineering
Ceramics and Composites
Biomaterials
Mechanics of Materials

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10.1016/j.biomaterials.2006.05.008

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title = "Practical application of a chromogenic FXIIa assay",

abstract = "Autohydrolysis of blood factor XII (FXII + FXIIa → 2 FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.",

author = "Rui Zhuo and Vogler, {Erwin A.}",

note = "Funding Information: This work was supported, in part, by the National Institute of Health PHS 5 R01 HL 69965-04, by Johnson & Johnson through the Focused Giving Grant Program, and a grant with the Pennsylvania Department of Health. The Department Pennsylvania Department of Health specifically disclaims responsibility for any analyses, interpretations or conclusions. Authors appreciate additional support from the Materials Research Institute and Departments of Bioengineering and Materials Science and Engineering, Penn State University.",

year = "2006",

month = oct,

doi = "10.1016/j.biomaterials.2006.05.008",

language = "English (US)",

volume = "27",

pages = "4840--4845",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "28",

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T1 - Practical application of a chromogenic FXIIa assay

AU - Zhuo, Rui

AU - Vogler, Erwin A.

N1 - Funding Information: This work was supported, in part, by the National Institute of Health PHS 5 R01 HL 69965-04, by Johnson & Johnson through the Focused Giving Grant Program, and a grant with the Pennsylvania Department of Health. The Department Pennsylvania Department of Health specifically disclaims responsibility for any analyses, interpretations or conclusions. Authors appreciate additional support from the Materials Research Institute and Departments of Bioengineering and Materials Science and Engineering, Penn State University.

PY - 2006/10

Y1 - 2006/10

N2 - Autohydrolysis of blood factor XII (FXII + FXIIa → 2 FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.

AB - Autohydrolysis of blood factor XII (FXII + FXIIa → 2 FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.

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U2 - 10.1016/j.biomaterials.2006.05.008

DO - 10.1016/j.biomaterials.2006.05.008

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AN - SCOPUS:33744992039

SN - 0142-9612

VL - 27

SP - 4840

EP - 4845

JO - Biomaterials

JF - Biomaterials

IS - 28

ER -

Practical application of a chromogenic FXIIa assay

Abstract

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