Predicted structural motif of ifnτ

Michael A. Jarpe, Howard M. Johnson, Fuller W. Bazer, Troy Ott, Ernie V. Curto, N. Rama Krishna, Carol H. Pontzer

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Ovine interferon Τ (IFNΤ) is a type I interferon that was originally identified as ovine trophoblast protein and is associated with the maternal recognition of pregnancy in sheep. Additionally, IFNΤ possesses potent antiviral and antiproliferative activity without the corresponding toxicity found in known IFNαs. Structure-function studies with synthetic peptides have identified three discontinuous functional sites on the protein that are involved in receptor interaction and biological activity. However, the structural relationship of these regions is unknown. Therefore, a model of the 3-D structure of IFNΤ would be useful in interpretation of existing data and the design of future structurefunction studies. Combining information from circular dichroism (CD) of both the full length recombinant IFNT and ynthetic peptides representing regions of the IFNΤ molecule, with sequence homology of IFNΤ to IFNβ ,a protein of known 3-D structure, we have constructed a model of IFNΤ using dis ance geometry and energy minimization methods. The most striking feature of this model is that functionally active domains of IFNΤ, discontinuous in the primary structure, are localized to one side of the molecule and found to be spatially contiguous. This observation is consistent with multiple binding sites on IFNT interacting simultaneously with the IFNΤ receptor.

Original languageEnglish (US)
Pages (from-to)863-867
Number of pages5
JournalProtein Engineering
Volume7
Issue number7
DOIs
StatePublished - Jan 1 1994

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Jarpe, M. A., Johnson, H. M., Bazer, F. W., Ott, T., Curto, E. V., Krishna, N. R., & Pontzer, C. H. (1994). Predicted structural motif of ifnτ. Protein Engineering, 7(7), 863-867. https://doi.org/10.1093/protein/7.7.863