Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system

Keisuke Yoshikawa, Shiro Takei, Sanae Ishii, Yoichi Chiba, Ayako Furukawa, Noriko Kawamura, Masanori Hosokawa, David F. Woodward, Kikuko Watanabe, Atsuyoshi Shimada

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Prostaglandin (PG) F is a product of cyclooxygenase (COX)-catalyzed metabolism of arachidonic acid and exerts biological functions in various tissues. Prostaglandin ethanolamide (prostamide) F is a COX-2-catalyzed metabolite of arachidonoyl ethanolamide (anandamide) that induces pharmacological actions in ocular tissues. Although PGF is one of the most abundant prostaglandins in the brain, function of PGF in the central nervous system (CNS) has not been extensively investigated. Recently identified prostamide/PGF synthase catalyzes the reductions of prostamide H2 to prostamide F and PGH2 to PGF, chiefly in the CNS. We examined tissue distribution of the enzyme in the CNS by immunohistochemistry, double immunofluorescence, and immuno-electron microscopy. We confirmed histological findings by immunofluorescence analyses of brain cell cultures. Prostamide/PGF synthase was expressed preferentially in the white matter bundles of the entire CNS of adult mice with less marked expression in neuronal cell bodies. The enzyme was colocalized with myelin basic protein (MBP) in myelin sheaths but not in axons. At the ultrastructural level, the enzyme was localized to myelin sheaths. Expression of the enzyme increased between P9 and P14 during the postnatal development, presumably in accordance with myelinogenesis. Cultured oligodendrocytes at 7 days in vitro expressed the enzyme in cytoplasmic processes where the enzyme was colocalized with MBP. Immunoreactivity for COX-2 was detected in white matter and cultured oligodendrocytes. Relatively selective localization of prostamide/PGF synthase suggests that myelin sheaths of the CNS may serve as the sites for producing prostamide F and/or PGF, which may contribute to the formation and maintenance of central myelin.

Original languageEnglish (US)
Pages (from-to)22-32
Number of pages11
JournalBrain research
Volume1367
DOIs
StatePublished - Jan 7 2011

Fingerprint

prostaglandin-F synthase
Dinoprost
Myelin Sheath
Prostaglandins
Central Nervous System
Enzymes
Prostaglandin H2
Myelin Basic Protein
Oligodendroglia
Prostaglandins F
Cyclooxygenase 2
Fluorescent Antibody Technique
Immunoelectron Microscopy
Brain
Tissue Distribution
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Axons
Cell Culture Techniques
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Yoshikawa, K., Takei, S., Ishii, S., Chiba, Y., Furukawa, A., Kawamura, N., ... Shimada, A. (2011). Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system. Brain research, 1367, 22-32. https://doi.org/10.1016/j.brainres.2010.10.019
Yoshikawa, Keisuke ; Takei, Shiro ; Ishii, Sanae ; Chiba, Yoichi ; Furukawa, Ayako ; Kawamura, Noriko ; Hosokawa, Masanori ; Woodward, David F. ; Watanabe, Kikuko ; Shimada, Atsuyoshi. / Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system. In: Brain research. 2011 ; Vol. 1367. pp. 22-32.
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title = "Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system",
abstract = "Prostaglandin (PG) F2α is a product of cyclooxygenase (COX)-catalyzed metabolism of arachidonic acid and exerts biological functions in various tissues. Prostaglandin ethanolamide (prostamide) F2α is a COX-2-catalyzed metabolite of arachidonoyl ethanolamide (anandamide) that induces pharmacological actions in ocular tissues. Although PGF 2α is one of the most abundant prostaglandins in the brain, function of PGF2α in the central nervous system (CNS) has not been extensively investigated. Recently identified prostamide/PGF synthase catalyzes the reductions of prostamide H2 to prostamide F 2α and PGH2 to PGF2α, chiefly in the CNS. We examined tissue distribution of the enzyme in the CNS by immunohistochemistry, double immunofluorescence, and immuno-electron microscopy. We confirmed histological findings by immunofluorescence analyses of brain cell cultures. Prostamide/PGF synthase was expressed preferentially in the white matter bundles of the entire CNS of adult mice with less marked expression in neuronal cell bodies. The enzyme was colocalized with myelin basic protein (MBP) in myelin sheaths but not in axons. At the ultrastructural level, the enzyme was localized to myelin sheaths. Expression of the enzyme increased between P9 and P14 during the postnatal development, presumably in accordance with myelinogenesis. Cultured oligodendrocytes at 7 days in vitro expressed the enzyme in cytoplasmic processes where the enzyme was colocalized with MBP. Immunoreactivity for COX-2 was detected in white matter and cultured oligodendrocytes. Relatively selective localization of prostamide/PGF synthase suggests that myelin sheaths of the CNS may serve as the sites for producing prostamide F2α and/or PGF2α, which may contribute to the formation and maintenance of central myelin.",
author = "Keisuke Yoshikawa and Shiro Takei and Sanae Ishii and Yoichi Chiba and Ayako Furukawa and Noriko Kawamura and Masanori Hosokawa and Woodward, {David F.} and Kikuko Watanabe and Atsuyoshi Shimada",
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Yoshikawa, K, Takei, S, Ishii, S, Chiba, Y, Furukawa, A, Kawamura, N, Hosokawa, M, Woodward, DF, Watanabe, K & Shimada, A 2011, 'Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system', Brain research, vol. 1367, pp. 22-32. https://doi.org/10.1016/j.brainres.2010.10.019

Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system. / Yoshikawa, Keisuke; Takei, Shiro; Ishii, Sanae; Chiba, Yoichi; Furukawa, Ayako; Kawamura, Noriko; Hosokawa, Masanori; Woodward, David F.; Watanabe, Kikuko; Shimada, Atsuyoshi.

In: Brain research, Vol. 1367, 07.01.2011, p. 22-32.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system

AU - Yoshikawa, Keisuke

AU - Takei, Shiro

AU - Ishii, Sanae

AU - Chiba, Yoichi

AU - Furukawa, Ayako

AU - Kawamura, Noriko

AU - Hosokawa, Masanori

AU - Woodward, David F.

AU - Watanabe, Kikuko

AU - Shimada, Atsuyoshi

PY - 2011/1/7

Y1 - 2011/1/7

N2 - Prostaglandin (PG) F2α is a product of cyclooxygenase (COX)-catalyzed metabolism of arachidonic acid and exerts biological functions in various tissues. Prostaglandin ethanolamide (prostamide) F2α is a COX-2-catalyzed metabolite of arachidonoyl ethanolamide (anandamide) that induces pharmacological actions in ocular tissues. Although PGF 2α is one of the most abundant prostaglandins in the brain, function of PGF2α in the central nervous system (CNS) has not been extensively investigated. Recently identified prostamide/PGF synthase catalyzes the reductions of prostamide H2 to prostamide F 2α and PGH2 to PGF2α, chiefly in the CNS. We examined tissue distribution of the enzyme in the CNS by immunohistochemistry, double immunofluorescence, and immuno-electron microscopy. We confirmed histological findings by immunofluorescence analyses of brain cell cultures. Prostamide/PGF synthase was expressed preferentially in the white matter bundles of the entire CNS of adult mice with less marked expression in neuronal cell bodies. The enzyme was colocalized with myelin basic protein (MBP) in myelin sheaths but not in axons. At the ultrastructural level, the enzyme was localized to myelin sheaths. Expression of the enzyme increased between P9 and P14 during the postnatal development, presumably in accordance with myelinogenesis. Cultured oligodendrocytes at 7 days in vitro expressed the enzyme in cytoplasmic processes where the enzyme was colocalized with MBP. Immunoreactivity for COX-2 was detected in white matter and cultured oligodendrocytes. Relatively selective localization of prostamide/PGF synthase suggests that myelin sheaths of the CNS may serve as the sites for producing prostamide F2α and/or PGF2α, which may contribute to the formation and maintenance of central myelin.

AB - Prostaglandin (PG) F2α is a product of cyclooxygenase (COX)-catalyzed metabolism of arachidonic acid and exerts biological functions in various tissues. Prostaglandin ethanolamide (prostamide) F2α is a COX-2-catalyzed metabolite of arachidonoyl ethanolamide (anandamide) that induces pharmacological actions in ocular tissues. Although PGF 2α is one of the most abundant prostaglandins in the brain, function of PGF2α in the central nervous system (CNS) has not been extensively investigated. Recently identified prostamide/PGF synthase catalyzes the reductions of prostamide H2 to prostamide F 2α and PGH2 to PGF2α, chiefly in the CNS. We examined tissue distribution of the enzyme in the CNS by immunohistochemistry, double immunofluorescence, and immuno-electron microscopy. We confirmed histological findings by immunofluorescence analyses of brain cell cultures. Prostamide/PGF synthase was expressed preferentially in the white matter bundles of the entire CNS of adult mice with less marked expression in neuronal cell bodies. The enzyme was colocalized with myelin basic protein (MBP) in myelin sheaths but not in axons. At the ultrastructural level, the enzyme was localized to myelin sheaths. Expression of the enzyme increased between P9 and P14 during the postnatal development, presumably in accordance with myelinogenesis. Cultured oligodendrocytes at 7 days in vitro expressed the enzyme in cytoplasmic processes where the enzyme was colocalized with MBP. Immunoreactivity for COX-2 was detected in white matter and cultured oligodendrocytes. Relatively selective localization of prostamide/PGF synthase suggests that myelin sheaths of the CNS may serve as the sites for producing prostamide F2α and/or PGF2α, which may contribute to the formation and maintenance of central myelin.

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