Prefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis

Ying Gu, Zhiping Deng, Alexander R. Paredez, Seth DeBolt, Zhi Yong Wang, Chris Somerville

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.

Original languageEnglish (US)
Pages (from-to)18064-18069
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number46
DOIs
StatePublished - Nov 18 2008

All Science Journal Classification (ASJC) codes

  • General

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