Preliminary amphibian health survey in the Delaware water Gap national recreation area

Gavin W. Glenney, James Thomas Julian, William M. Quartz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To detect aquatic animal diseases of national concern, 111 individual amphibians, including wood frogs Rana sylvatica (28), spring peepers Pseudacris crucifer (35), red-spotted newts Notophthalmus viridescens (41), and gray tree frogs Hyla versicolor (7), were sampled at seven different sites in the Delaware Water Gap National Recreation Area (DGNRA), Pennsylvania, from June 14 to July 19, 2007. These samples were screened for Batrachochytrium dendrobatidis and viral pathogens at the U.S. Fish and Wildlife Service's Fish Health Center in Lamar, Pennsylvania. Cell culture revealed cytopathic effect (CPE) in two cell lines (epithelioma papillosum cyprini and fathead minnow) inoculated with liver, kidney, and spleen samples from one sample pool of Notophthalmus viridescens (4 individuals). Polymerase chain reaction was conducted on cell culture supernatant exhibiting CPE. Sequencing revealed the resulting product to be identical to frog virus 3, a ranavirus in the family Iridoviridae. Upon gross examination, two Notophthalmus viridescens were found to exhibit dermal swelling and lethargy. Histological examination of these lesions revealed involvement by an Ichthyophonus sp. In summary, two pathogens of concern were found in amphibians in the DGNRA: a ranavirus with a major capsid protein sequence identical to that of frog virus 3 and a mesomycetozoan, Ichthyophonus sp. Although no epizootic die-offs were observed during this health survey, the results warrant further research into the distribution of these pathogens throughout the DGNRA because they have the potential to cause mass mortalities in amphibians.

Original languageEnglish (US)
Pages (from-to)102-114
Number of pages13
JournalJournal of Aquatic Animal Health
Volume22
Issue number2
DOIs
StatePublished - Dec 1 2010

Fingerprint

Notophthalmus viridescens
recreation areas
health survey
Frog virus 3
Ichthyophonus
Ranavirus
frog
amphibian
amphibians
cytopathogenicity
pathogen
pathogens
cell culture
Iridoviridae
Hyla versicolor
U.S. Fish and Wildlife Service
Pimephales promelas
fish health
virus
water

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Glenney, Gavin W. ; Julian, James Thomas ; Quartz, William M. / Preliminary amphibian health survey in the Delaware water Gap national recreation area. In: Journal of Aquatic Animal Health. 2010 ; Vol. 22, No. 2. pp. 102-114.
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Preliminary amphibian health survey in the Delaware water Gap national recreation area. / Glenney, Gavin W.; Julian, James Thomas; Quartz, William M.

In: Journal of Aquatic Animal Health, Vol. 22, No. 2, 01.12.2010, p. 102-114.

Research output: Contribution to journalArticle

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