Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase

E. A. Stura, D. L. Johnson, J. Inglese, J. M. Smith, S. J. Benkovic, I. A. Wilson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 Å, b = 98.2 Å, c = 103.5 Å. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (K(i) = 18 μM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (<2.5 Å) x-ray diffraction studies have been obtained for each of the above.

Original languageEnglish (US)
Pages (from-to)9703-9706
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number16
StatePublished - Jan 1 1989

Fingerprint

Phosphoribosylglycinamide Formyltransferase
Crystals
Ammonium Sulfate
Enzymes
Crystallization
Citric Acid
X-Rays
Diffraction
Sodium
Single crystals
X rays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Stura, E. A., Johnson, D. L., Inglese, J., Smith, J. M., Benkovic, S. J., & Wilson, I. A. (1989). Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase. Journal of Biological Chemistry, 264(16), 9703-9706.
Stura, E. A. ; Johnson, D. L. ; Inglese, J. ; Smith, J. M. ; Benkovic, S. J. ; Wilson, I. A. / Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 16. pp. 9703-9706.
@article{3f4ec34388dc4f68897529e3a7b1fcfb,
title = "Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase",
abstract = "Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 {\AA}, b = 98.2 {\AA}, c = 103.5 {\AA}. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (K(i) = 18 μM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (<2.5 {\AA}) x-ray diffraction studies have been obtained for each of the above.",
author = "Stura, {E. A.} and Johnson, {D. L.} and J. Inglese and Smith, {J. M.} and Benkovic, {S. J.} and Wilson, {I. A.}",
year = "1989",
month = "1",
day = "1",
language = "English (US)",
volume = "264",
pages = "9703--9706",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

Stura, EA, Johnson, DL, Inglese, J, Smith, JM, Benkovic, SJ & Wilson, IA 1989, 'Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase', Journal of Biological Chemistry, vol. 264, no. 16, pp. 9703-9706.

Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase. / Stura, E. A.; Johnson, D. L.; Inglese, J.; Smith, J. M.; Benkovic, S. J.; Wilson, I. A.

In: Journal of Biological Chemistry, Vol. 264, No. 16, 01.01.1989, p. 9703-9706.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase

AU - Stura, E. A.

AU - Johnson, D. L.

AU - Inglese, J.

AU - Smith, J. M.

AU - Benkovic, S. J.

AU - Wilson, I. A.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 Å, b = 98.2 Å, c = 103.5 Å. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (K(i) = 18 μM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (<2.5 Å) x-ray diffraction studies have been obtained for each of the above.

AB - Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 Å, b = 98.2 Å, c = 103.5 Å. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (K(i) = 18 μM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (<2.5 Å) x-ray diffraction studies have been obtained for each of the above.

UR - http://www.scopus.com/inward/record.url?scp=0024308085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024308085&partnerID=8YFLogxK

M3 - Article

C2 - 2656702

AN - SCOPUS:0024308085

VL - 264

SP - 9703

EP - 9706

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -

Stura EA, Johnson DL, Inglese J, Smith JM, Benkovic SJ, Wilson IA. Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase. Journal of Biological Chemistry. 1989 Jan 1;264(16):9703-9706.