Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase

E. A. Stura, D. L. Johnson, J. Inglese, J. M. Smith, S. J. Benkovic, I. A. Wilson

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Abstract

Crystals of glycinamide ribonucleotide transformylase have been grown from 0.4 to 1 M ammonium sulfate, 0.6 to 1 M sodium-potassium phosphate, or 0.65 to 1 M citrate in the pH range 4.5-7.0. The single crystals display variable morphology with varying pH. The crystals belong to the orthorhombic space group C222 with cell dimensions a = 141.4 Å, b = 98.2 Å, c = 103.5 Å. Co-crystals have also been obtained in the presence of the inhibitor 5,8-dideazafolate (K(i) = 18 μM) under similar crystallization conditions. Crystals of a chemically modified enzyme, iodinated at Cys-21, were grown under similar conditions within the pH range 6.5-7.0. These crystals are isomorphous with the unmodified enzyme. Crystals suitable for high resolution (<2.5 Å) x-ray diffraction studies have been obtained for each of the above.

Original languageEnglish (US)
Pages (from-to)9703-9706
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number16
StatePublished - Jan 1 1989

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Stura, E. A., Johnson, D. L., Inglese, J., Smith, J. M., Benkovic, S. J., & Wilson, I. A. (1989). Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase. Journal of Biological Chemistry, 264(16), 9703-9706.