Preliminary Investigation of the Changes in Fecal Streptococcal Population due to Diet and Time of Day in Horses

William Burton Staniar, Lauren E. Neuendorf, Samantha A. Brooks

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A growing number of equine studies examine management factors that influence the microbial populations in regions of the gastrointestinal tract using culture-independent molecular techniques. We used quantitative polymerase chain reaction (qPCR) to evaluate changes in fecal streptococcal populations in horses fed different maturities of teff hay with fecal samples collected at 7 AM, 12 PM, and 7 PM. The objective of this study was to use qPCR and species-specific probes for 16S ribosomal DNA to quantify the percentage of equine hindgut streptococcal species (EHSS) relative to the total bacterial load in the feces. Feces from horses fed the most mature teff hay had the lowest %EHSS, and feces collected at 12:00 had the highest %EHSS (P < .05). Our interest in investigating %EHSS developed out of research that examined associations between changes in EHSS populations and the onset of laminitis. Although we expected almost no risk of laminitis in these forage fed horses, our hypothesis was that the different carbohydrate fractions in the three maturities of hay would result in differences in %EHSS in horses fed these hay maturities. The supply of carbohydrates, nonstructural or structural, influences the microbial species composition through the gastrointestinal tract. This study highlights the occurrence of measurable changes in %EHSS due to subtle changes in dietary nonstructural carbohydrate and also changes in samples taken at different times of day. This information is useful to others considering investigations of dietary influences on gastrointestinal microbial populations, particularly those that plan to use feces as their main sample medium.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalJournal of Equine Veterinary Science
Volume46
DOIs
StatePublished - Nov 1 2016

Fingerprint

Horses
Diet
horses
hindgut
diet
Population
Feces
hay
Eragrostis
feces
Eragrostis tef
laminitis
carbohydrates
gastrointestinal system
Gastrointestinal Tract
quantitative polymerase chain reaction
Carbohydrates
Dietary Carbohydrates
Polymerase Chain Reaction
Bacterial Load

All Science Journal Classification (ASJC) codes

  • Equine

Cite this

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title = "Preliminary Investigation of the Changes in Fecal Streptococcal Population due to Diet and Time of Day in Horses",
abstract = "A growing number of equine studies examine management factors that influence the microbial populations in regions of the gastrointestinal tract using culture-independent molecular techniques. We used quantitative polymerase chain reaction (qPCR) to evaluate changes in fecal streptococcal populations in horses fed different maturities of teff hay with fecal samples collected at 7 AM, 12 PM, and 7 PM. The objective of this study was to use qPCR and species-specific probes for 16S ribosomal DNA to quantify the percentage of equine hindgut streptococcal species (EHSS) relative to the total bacterial load in the feces. Feces from horses fed the most mature teff hay had the lowest {\%}EHSS, and feces collected at 12:00 had the highest {\%}EHSS (P < .05). Our interest in investigating {\%}EHSS developed out of research that examined associations between changes in EHSS populations and the onset of laminitis. Although we expected almost no risk of laminitis in these forage fed horses, our hypothesis was that the different carbohydrate fractions in the three maturities of hay would result in differences in {\%}EHSS in horses fed these hay maturities. The supply of carbohydrates, nonstructural or structural, influences the microbial species composition through the gastrointestinal tract. This study highlights the occurrence of measurable changes in {\%}EHSS due to subtle changes in dietary nonstructural carbohydrate and also changes in samples taken at different times of day. This information is useful to others considering investigations of dietary influences on gastrointestinal microbial populations, particularly those that plan to use feces as their main sample medium.",
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Preliminary Investigation of the Changes in Fecal Streptococcal Population due to Diet and Time of Day in Horses. / Staniar, William Burton; Neuendorf, Lauren E.; Brooks, Samantha A.

In: Journal of Equine Veterinary Science, Vol. 46, 01.11.2016, p. 18-23.

Research output: Contribution to journalArticle

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