Preliminary study on regulable DNA vaccines against Plasmodium falciparum

Jun chuan Lei, Jun Miao, Xun Li, Kai Schonig, Hermann Bujard, Cai fang Xue

Research output: Contribution to journalArticle

Abstract

AIM: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system. METHODS: Eukaryotic expression vectors pTL-8/apical membrane antigen 1 (AMA-1) (tTA) and pTL-8/AMA-1(rtTA) gene which express trans-activator (tTA) or reverse trans-activator(rtTA), respectively, and AMA-1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA-1. For some mice immunized with pTL-8/AMA-1(rtTA), pUHS6-1, a plasmid containing trans-silencer (tTS) to suppress basal expression of AMA-1 from pTL-8/AMA-1(rtTA), was injected into these mice together with pTL-8/AMA-1(rtTA). The sera of the mice were isolated at 2,4,6 and 8 weeks post-immunization and the antibodies specific to AMA-1 were measured by ELISA. RESULTS: pTL-8/AMA-1 and pTL-8/AMA-1(rtTA) were constructed successfully. The mice immunized by pTL-8/AMA-1(tTA) with dox or by pTL-8/AMA-1(rtTA) without dox (at these conditions, AMA-1 was expressed at basal level)developed significant antibodies against AMA-1. Mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 without dox did not develop significantly antibodies against AMA-1. In contrast, the mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 with dox produced high level of antibodies. CONCLUSION: pTL-8/AMA-1(rtTA) combined with pUHS6-1 is a good regulable DNA vaccine candidate against Plasmodium falciparum.

Original languageEnglish (US)
Pages (from-to)356-359
Number of pages4
JournalXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Volume20
Issue number3
StatePublished - Jan 1 2004

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DNA Vaccines
Plasmodium falciparum
Trans-Activators
Antigens
Membranes
Doxycycline
Antibodies
Plasmids

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lei, Jun chuan ; Miao, Jun ; Li, Xun ; Schonig, Kai ; Bujard, Hermann ; Xue, Cai fang. / Preliminary study on regulable DNA vaccines against Plasmodium falciparum. In: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2004 ; Vol. 20, No. 3. pp. 356-359.
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title = "Preliminary study on regulable DNA vaccines against Plasmodium falciparum",
abstract = "AIM: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system. METHODS: Eukaryotic expression vectors pTL-8/apical membrane antigen 1 (AMA-1) (tTA) and pTL-8/AMA-1(rtTA) gene which express trans-activator (tTA) or reverse trans-activator(rtTA), respectively, and AMA-1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA-1. For some mice immunized with pTL-8/AMA-1(rtTA), pUHS6-1, a plasmid containing trans-silencer (tTS) to suppress basal expression of AMA-1 from pTL-8/AMA-1(rtTA), was injected into these mice together with pTL-8/AMA-1(rtTA). The sera of the mice were isolated at 2,4,6 and 8 weeks post-immunization and the antibodies specific to AMA-1 were measured by ELISA. RESULTS: pTL-8/AMA-1 and pTL-8/AMA-1(rtTA) were constructed successfully. The mice immunized by pTL-8/AMA-1(tTA) with dox or by pTL-8/AMA-1(rtTA) without dox (at these conditions, AMA-1 was expressed at basal level)developed significant antibodies against AMA-1. Mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 without dox did not develop significantly antibodies against AMA-1. In contrast, the mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 with dox produced high level of antibodies. CONCLUSION: pTL-8/AMA-1(rtTA) combined with pUHS6-1 is a good regulable DNA vaccine candidate against Plasmodium falciparum.",
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Preliminary study on regulable DNA vaccines against Plasmodium falciparum. / Lei, Jun chuan; Miao, Jun; Li, Xun; Schonig, Kai; Bujard, Hermann; Xue, Cai fang.

In: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, Vol. 20, No. 3, 01.01.2004, p. 356-359.

Research output: Contribution to journalArticle

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AU - Lei, Jun chuan

AU - Miao, Jun

AU - Li, Xun

AU - Schonig, Kai

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N2 - AIM: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system. METHODS: Eukaryotic expression vectors pTL-8/apical membrane antigen 1 (AMA-1) (tTA) and pTL-8/AMA-1(rtTA) gene which express trans-activator (tTA) or reverse trans-activator(rtTA), respectively, and AMA-1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA-1. For some mice immunized with pTL-8/AMA-1(rtTA), pUHS6-1, a plasmid containing trans-silencer (tTS) to suppress basal expression of AMA-1 from pTL-8/AMA-1(rtTA), was injected into these mice together with pTL-8/AMA-1(rtTA). The sera of the mice were isolated at 2,4,6 and 8 weeks post-immunization and the antibodies specific to AMA-1 were measured by ELISA. RESULTS: pTL-8/AMA-1 and pTL-8/AMA-1(rtTA) were constructed successfully. The mice immunized by pTL-8/AMA-1(tTA) with dox or by pTL-8/AMA-1(rtTA) without dox (at these conditions, AMA-1 was expressed at basal level)developed significant antibodies against AMA-1. Mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 without dox did not develop significantly antibodies against AMA-1. In contrast, the mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 with dox produced high level of antibodies. CONCLUSION: pTL-8/AMA-1(rtTA) combined with pUHS6-1 is a good regulable DNA vaccine candidate against Plasmodium falciparum.

AB - AIM: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system. METHODS: Eukaryotic expression vectors pTL-8/apical membrane antigen 1 (AMA-1) (tTA) and pTL-8/AMA-1(rtTA) gene which express trans-activator (tTA) or reverse trans-activator(rtTA), respectively, and AMA-1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA-1. For some mice immunized with pTL-8/AMA-1(rtTA), pUHS6-1, a plasmid containing trans-silencer (tTS) to suppress basal expression of AMA-1 from pTL-8/AMA-1(rtTA), was injected into these mice together with pTL-8/AMA-1(rtTA). The sera of the mice were isolated at 2,4,6 and 8 weeks post-immunization and the antibodies specific to AMA-1 were measured by ELISA. RESULTS: pTL-8/AMA-1 and pTL-8/AMA-1(rtTA) were constructed successfully. The mice immunized by pTL-8/AMA-1(tTA) with dox or by pTL-8/AMA-1(rtTA) without dox (at these conditions, AMA-1 was expressed at basal level)developed significant antibodies against AMA-1. Mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 without dox did not develop significantly antibodies against AMA-1. In contrast, the mice immunized by pTL-8/AMA-1(rtTA) and pUHS6-1 with dox produced high level of antibodies. CONCLUSION: pTL-8/AMA-1(rtTA) combined with pUHS6-1 is a good regulable DNA vaccine candidate against Plasmodium falciparum.

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