Preliminary use of differential scanning calorimetry of cerebrospinal fluid for the diagnosis of glioblastoma multiforme

Alexis A. Chagovetz, Randy L. Jensen, Larry Recht, Michael Glantz, Alexander M. Chagovetz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Thermal stability signatures of complex molecule interaction in biological fluids can be measured using a new approach called differential scanning calorimetry (DSC). The thermal stability of plasma proteome has been described previously as a method of producing a disease-specific "signature," termed thermogram, in several neoplastic and autoimmune diseases. We describe the preliminary use of DSC performed on cerebrospinal fluid (CSF) as a diagnostic tool for the identification of patients with glioblastoma multiforme (GBM). Samples of CSF from nine patients with confirmed GBM were evaluated using DSC, and the thermogram signatures evaluated. These thermograms were compared with thermograms of CSF taken from patients with non-neoplastic conditions such as head trauma, hydrocephalus, or CSF leak. Further analysis was also performed on CSF from patients who had non-GBM neoplastic conditions such as carcinomatosis meningitis or central nervous system lymphoma or leukemia. The DSC thermograms of CSF of the patients with GBM were significantly different when compared with other neoplastic and non-neoplastic cases. The melting temperature of the major transition was shifted by 5°C, which makes it easily distinguishable from control cases. Our results are very preliminary, but it appears that the DSC of CSF has potential utility in diagnostics and monitoring disease progression in GBM patients.

Original languageEnglish (US)
Pages (from-to)499-506
Number of pages8
JournalJournal of neuro-oncology
Volume105
Issue number3
DOIs
StatePublished - Dec 1 2011

Fingerprint

Differential Scanning Calorimetry
Glioblastoma
Cerebrospinal Fluid
Plasma Gases
Transition Temperature
Proteome
Hydrocephalus
Craniocerebral Trauma
Meningitis
Freezing
Autoimmune Diseases
Disease Progression
Lymphoma
Leukemia
Central Nervous System
Hot Temperature
Carcinoma

All Science Journal Classification (ASJC) codes

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Cite this

Chagovetz, Alexis A. ; Jensen, Randy L. ; Recht, Larry ; Glantz, Michael ; Chagovetz, Alexander M. / Preliminary use of differential scanning calorimetry of cerebrospinal fluid for the diagnosis of glioblastoma multiforme. In: Journal of neuro-oncology. 2011 ; Vol. 105, No. 3. pp. 499-506.
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Preliminary use of differential scanning calorimetry of cerebrospinal fluid for the diagnosis of glioblastoma multiforme. / Chagovetz, Alexis A.; Jensen, Randy L.; Recht, Larry; Glantz, Michael; Chagovetz, Alexander M.

In: Journal of neuro-oncology, Vol. 105, No. 3, 01.12.2011, p. 499-506.

Research output: Contribution to journalArticle

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