Preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles

Xi Jun Chen, Robert J. Hickey, So Jung Park

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We have recently developed a universal procedure to functionalize inorganic nanoparticles with a dense layer of DNA through the self-assembly of DNA block copolymers and nanoparticles. This functionalization strategy allows one to combine the useful physical properties of inorganic nanoparticle with the enhanced DNA binding properties that originate from the high surface DNA density. In particular, the hybrid nanostructures exhibit orders of magnitude higher binding constants than regular DNA strands. This chapter presents a detailed protocol for the preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles.

Original languageEnglish (US)
Title of host publicationNanomaterial Interfaces in Biology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages207-224
Number of pages18
ISBN (Print)9781627034616
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1025
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Chen, X. J., Hickey, R. J., & Park, S. J. (2013). Preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles. In Nanomaterial Interfaces in Biology: Methods and Protocols (pp. 207-224). (Methods in Molecular Biology; Vol. 1025). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-462-3_16