Preparation of magnetic graphene composites with hierarchical structure for selective capture of phosphopeptides

Gong Cheng, Xu Yu, Ming Da Zhou, Si Yang Zheng

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A novel graphene composite affinity material consisting of graphene scaffolds, Fe3O4 nanoparticles for actuation and fully covered porous titania nanostructures as affinity coating has been designed and constructed. The obtained magnetic graphene composites have a saturation magnetization (Ms) value of 7.3 emu g-1, a BET specific surface area of 111.8 m2 g-1 and an average pore size of 15.1 nm for the porous affinity coating. The multifunctional graphene composites can realize the selective capture and convenient magnetic separation of target phosphopeptides by taking advantage of the decorated magnetic nanoparticles, highly pure and well crystallized affinity coating, and unique porous structure. The sensitivity and selectivity of the affinity graphene composites were evaluated using digests of standard proteins and complex biosamples as well as by comparison with the widely used TiO2 affinity microspheres. The results show that the affinity graphene composites can realize selective capture and rapid separation of low-abundant phosphopeptides from complex biological samples. Thus, this work will contribute to future applications in the purification and separation of specific biomolecules, in particular low-abundant phosphopeptide biomarkers.

Original languageEnglish (US)
Pages (from-to)4711-4719
Number of pages9
JournalJournal of Materials Chemistry B
Volume2
Issue number29
DOIs
StatePublished - Aug 7 2014

Fingerprint

Phosphopeptides
Graphite
Graphene
Composite materials
Coatings
Nanoparticles
Dromaiidae
Magnetic separation
Nanostructures
Biomolecules
Biomarkers
Saturation magnetization
Microspheres
Scaffolds
Specific surface area
Pore size
Purification
Titanium
Proteins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Cheng, Gong ; Yu, Xu ; Zhou, Ming Da ; Zheng, Si Yang. / Preparation of magnetic graphene composites with hierarchical structure for selective capture of phosphopeptides. In: Journal of Materials Chemistry B. 2014 ; Vol. 2, No. 29. pp. 4711-4719.
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Preparation of magnetic graphene composites with hierarchical structure for selective capture of phosphopeptides. / Cheng, Gong; Yu, Xu; Zhou, Ming Da; Zheng, Si Yang.

In: Journal of Materials Chemistry B, Vol. 2, No. 29, 07.08.2014, p. 4711-4719.

Research output: Contribution to journalArticle

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