Preparation of tunable microchips to visualize native protein complexes for single-particle electron microscopy

Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes, Deborah F. Kelly

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining monolayer purification with silicon nitride microchips to create a tunable approach for the EM community.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages45-58
Number of pages14
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1764
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Gilmore, B. L., Varano, A. C., Dearnaley, W., Liang, Y., Marcinkowski, B. C., Dukes, M. J., & Kelly, D. F. (2018). Preparation of tunable microchips to visualize native protein complexes for single-particle electron microscopy. In Methods in Molecular Biology (pp. 45-58). (Methods in Molecular Biology; Vol. 1764). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7759-8_3