ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pKa of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.
|Original language||English (US)|
|Number of pages||18|
|Journal||Journal of Food Biochemistry|
|State||Published - Dec 1992|
All Science Journal Classification (ASJC) codes
- Food Science
- Cell Biology