PREPARATION, PURIFICATION AND IDENTIFICATION OF 10‐OXO‐TRANS‐8‐DECENOIC ACID FROM THE CULTIVATED MUSHROOM, AGARICUS BISPORUS

JENG‐LEUN ‐L MAU, ROBERT B. BEELMAN, GREGORY R. ZIEGLER

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3 Scopus citations

Abstract

ABSTRACT A procedure was developed to isolate 10‐oxo‐trans‐8‐decenoic acid (ODA) from mushrooms. ODA was produced by homogenizing mushrooms in phosphate buffer with added linoleic acid, extracted from the supernatant after centrifugation, and purified using column and thin‐layer chromatography. The purified compound was then characterized using ultraviolet, infrared and mass spectrometry, and nuclear magnetic resonance spectroscopy. The purified compound, containing 97.5% ODA, was a white, waxy solid with a pKa of 4.68. ODA was soluble in acetone, chloroform, ethanol, ethyl ether, methanol, methylene chloride and water, and slightly soluble in pentane, hexane, heptane and benzene. The TBA test was found to be a viable method for the quantification of ODA.

Original languageEnglish (US)
Pages (from-to)371-388
Number of pages18
JournalJournal of Food Biochemistry
Volume16
Issue number6
DOIs
StatePublished - Dec 1992

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

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