Presence of viral and bacterial organisms in milk and their association with somatic cell counts

D. A. Herlekar, Cooduvalli S. Shashikant, A. A. Gurjar, Bhushan M. Jayarao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

About 20 to 35% of milk samples from cows with intramammary infection or high somatic cell count (SCC) are negative on bacteriological culture analysis. However, little is known about SCC in milk of cows infected with viruses. In the first part of our study, we developed a real-time PCR assay for detection of bovine herpesvirus (BHV) 1, BHV2, and BHV4, and bovine viral diarrhea virus (BVDV) in composite quarter milk samples. A total of 1,479 lactating cows of 1,964 cows in the dairy herd were initially selected because these cows had complete SCC data for at least 3 consecutive test results, of which 139 lactating cows from different lactation age groups were selected randomly and studied extensively. Composite quarter milk samples were collected on 3 alternate days and examined for viruses, SCC, and bacteriological analysis. In total, 10, 28, and 0.7% of the composite quarter milk samples from cows were positive for BHV1, BHV2, and BHV4, respectively; BVDV was not detected in composite quarter milk samples. Bovine herpesvirus was not associated with a particular bacterial species. Our study results indicate that cows positive for BHV in composite quarter milk samples alone are less likely to have elevated SCC compared with cows with bacterial intramammary infection; BHV1, BHV2, and BHV4 are probably not major udder pathogens.

Original languageEnglish (US)
Pages (from-to)6336-6346
Number of pages11
JournalJournal of dairy science
Volume96
Issue number10
DOIs
StatePublished - Oct 1 2013

Fingerprint

somatic cell count
Milk
Cell Count
cows
milk
organisms
Bovine Viral Diarrhea Viruses
Herpesviridae
Bovine viral diarrhea virus
sampling
Bovine Herpesvirus 1
Viruses
Animal Mammary Glands
viruses
Bovine herpesvirus 1
cattle
Lactation
Bacterial Infections
bacterial infections
udders

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

@article{725788fe8beb49cc9dadfeb0d931128a,
title = "Presence of viral and bacterial organisms in milk and their association with somatic cell counts",
abstract = "About 20 to 35{\%} of milk samples from cows with intramammary infection or high somatic cell count (SCC) are negative on bacteriological culture analysis. However, little is known about SCC in milk of cows infected with viruses. In the first part of our study, we developed a real-time PCR assay for detection of bovine herpesvirus (BHV) 1, BHV2, and BHV4, and bovine viral diarrhea virus (BVDV) in composite quarter milk samples. A total of 1,479 lactating cows of 1,964 cows in the dairy herd were initially selected because these cows had complete SCC data for at least 3 consecutive test results, of which 139 lactating cows from different lactation age groups were selected randomly and studied extensively. Composite quarter milk samples were collected on 3 alternate days and examined for viruses, SCC, and bacteriological analysis. In total, 10, 28, and 0.7{\%} of the composite quarter milk samples from cows were positive for BHV1, BHV2, and BHV4, respectively; BVDV was not detected in composite quarter milk samples. Bovine herpesvirus was not associated with a particular bacterial species. Our study results indicate that cows positive for BHV in composite quarter milk samples alone are less likely to have elevated SCC compared with cows with bacterial intramammary infection; BHV1, BHV2, and BHV4 are probably not major udder pathogens.",
author = "Herlekar, {D. A.} and Shashikant, {Cooduvalli S.} and Gurjar, {A. A.} and Jayarao, {Bhushan M.}",
year = "2013",
month = "10",
day = "1",
doi = "10.3168/jds.2013-6631",
language = "English (US)",
volume = "96",
pages = "6336--6346",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "10",

}

Presence of viral and bacterial organisms in milk and their association with somatic cell counts. / Herlekar, D. A.; Shashikant, Cooduvalli S.; Gurjar, A. A.; Jayarao, Bhushan M.

In: Journal of dairy science, Vol. 96, No. 10, 01.10.2013, p. 6336-6346.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Presence of viral and bacterial organisms in milk and their association with somatic cell counts

AU - Herlekar, D. A.

AU - Shashikant, Cooduvalli S.

AU - Gurjar, A. A.

AU - Jayarao, Bhushan M.

PY - 2013/10/1

Y1 - 2013/10/1

N2 - About 20 to 35% of milk samples from cows with intramammary infection or high somatic cell count (SCC) are negative on bacteriological culture analysis. However, little is known about SCC in milk of cows infected with viruses. In the first part of our study, we developed a real-time PCR assay for detection of bovine herpesvirus (BHV) 1, BHV2, and BHV4, and bovine viral diarrhea virus (BVDV) in composite quarter milk samples. A total of 1,479 lactating cows of 1,964 cows in the dairy herd were initially selected because these cows had complete SCC data for at least 3 consecutive test results, of which 139 lactating cows from different lactation age groups were selected randomly and studied extensively. Composite quarter milk samples were collected on 3 alternate days and examined for viruses, SCC, and bacteriological analysis. In total, 10, 28, and 0.7% of the composite quarter milk samples from cows were positive for BHV1, BHV2, and BHV4, respectively; BVDV was not detected in composite quarter milk samples. Bovine herpesvirus was not associated with a particular bacterial species. Our study results indicate that cows positive for BHV in composite quarter milk samples alone are less likely to have elevated SCC compared with cows with bacterial intramammary infection; BHV1, BHV2, and BHV4 are probably not major udder pathogens.

AB - About 20 to 35% of milk samples from cows with intramammary infection or high somatic cell count (SCC) are negative on bacteriological culture analysis. However, little is known about SCC in milk of cows infected with viruses. In the first part of our study, we developed a real-time PCR assay for detection of bovine herpesvirus (BHV) 1, BHV2, and BHV4, and bovine viral diarrhea virus (BVDV) in composite quarter milk samples. A total of 1,479 lactating cows of 1,964 cows in the dairy herd were initially selected because these cows had complete SCC data for at least 3 consecutive test results, of which 139 lactating cows from different lactation age groups were selected randomly and studied extensively. Composite quarter milk samples were collected on 3 alternate days and examined for viruses, SCC, and bacteriological analysis. In total, 10, 28, and 0.7% of the composite quarter milk samples from cows were positive for BHV1, BHV2, and BHV4, respectively; BVDV was not detected in composite quarter milk samples. Bovine herpesvirus was not associated with a particular bacterial species. Our study results indicate that cows positive for BHV in composite quarter milk samples alone are less likely to have elevated SCC compared with cows with bacterial intramammary infection; BHV1, BHV2, and BHV4 are probably not major udder pathogens.

UR - http://www.scopus.com/inward/record.url?scp=84884352949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884352949&partnerID=8YFLogxK

U2 - 10.3168/jds.2013-6631

DO - 10.3168/jds.2013-6631

M3 - Article

C2 - 23972495

AN - SCOPUS:84884352949

VL - 96

SP - 6336

EP - 6346

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 10

ER -