TY - JOUR
T1 - Prevalence of Campylobacter species and associated risk factors for contamination of dairy products collected in a dry season from major milk sheds in Ethiopia
AU - Admasie, Abera
AU - Eshetu, Adane
AU - Tessema, Tesfaye Sisay
AU - Vipham, Jessie
AU - Kovac, Jasna
AU - Zewdu, Ashagrie
N1 - Funding Information:
This work was supported, in whole or in part, by the Bill & Melinda Gates Foundation and the UK Foreign, Commonwealth, and Development Office, grant number INV-008459. Under the grant conditions of the Foundation, a Creative Commons Attribution 4.0 Generic License has already been assigned to the Author Accepted Manuscript version that might arise from this submission. JK was partially funded by the USDA National Institute of Food and Agriculture , Hatch Appropriations under Project #PEN04646 and Accession #1015787. We would like to thank the Oromia, Amhara, and SNNP research participants for their willingness to participate in the survey. We would also like to thank the agricultural development agents at each study site for their assistance with the subject identification and enrollment, as well as questionnaire administration. Thank you also to the Department of Microbiology personnel of the Ethiopian Conformity and Assessment Enterprise for their great technical and moral assistance during the laboratory analyses.
Funding Information:
This work was supported, in whole or in part, by the Bill & Melinda Gates Foundation and the UK Foreign, Commonwealth, and Development Office, grant number INV-008459. Under the grant conditions of the Foundation, a Creative Commons Attribution 4.0 Generic License has already been assigned to the Author Accepted Manuscript version that might arise from this submission. JK was partially funded by the USDA National Institute of Food and Agriculture, Hatch Appropriations under Project #PEN04646 and Accession #1015787. We would like to thank the Oromia, Amhara, and SNNP research participants for their willingness to participate in the survey. We would also like to thank the agricultural development agents at each study site for their assistance with the subject identification and enrollment, as well as questionnaire administration. Thank you also to the Department of Microbiology personnel of the Ethiopian Conformity and Assessment Enterprise for their great technical and moral assistance during the laboratory analyses.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2023/2
Y1 - 2023/2
N2 - A cross-sectional study was conducted to investigate the prevalence and risk factors for contamination of Ethiopian dairy products with Campylobacter. A total of 912 dairy food samples were collected from establishments of 682 study participants that were interviewed. Samples were tested for Campylobacter by following the ISO 10272–1:2017 standard and PCR confirmation. Campylobacter was detected in 11% of tested food samples and all detected Campylobacter were C. jejuni. The highest prevalence of C. jejuni was found in raw milk (16%), followed by pasteurized milk (9%) and cottage cheese (2%) (P < 0.001). Using warm water and soap for cleaning cow udders and teats on farms reduced the likelihood of detecting Campylobacter in milk (AOR = 0.3, P = 0.023). Filtering milk with a cloth, using a plastic filter (AOR = 0.065, P = 0.005), and storing milk in an aluminum container (AOR = 0.23, P = 0.027) reduced the likelihood of detecting Campylobacter in milk at the collection facilities. In contrast, Campylobacter detection was significantly more likely in milk collected at collection centers with concrete floors (AOR = 5.2, P = 0.004). The odds of detecting Campylobacter in milk were 17 times greater (AOR = 17, P = 0.007) in milk processing facilities that did not calibrate a pasteurizer on an annual basis. Finally, having a separate refrigerator for milk storage reduced the odds of detecting Campylobacter in retail (AOR = 0.29, P = 0.021).
AB - A cross-sectional study was conducted to investigate the prevalence and risk factors for contamination of Ethiopian dairy products with Campylobacter. A total of 912 dairy food samples were collected from establishments of 682 study participants that were interviewed. Samples were tested for Campylobacter by following the ISO 10272–1:2017 standard and PCR confirmation. Campylobacter was detected in 11% of tested food samples and all detected Campylobacter were C. jejuni. The highest prevalence of C. jejuni was found in raw milk (16%), followed by pasteurized milk (9%) and cottage cheese (2%) (P < 0.001). Using warm water and soap for cleaning cow udders and teats on farms reduced the likelihood of detecting Campylobacter in milk (AOR = 0.3, P = 0.023). Filtering milk with a cloth, using a plastic filter (AOR = 0.065, P = 0.005), and storing milk in an aluminum container (AOR = 0.23, P = 0.027) reduced the likelihood of detecting Campylobacter in milk at the collection facilities. In contrast, Campylobacter detection was significantly more likely in milk collected at collection centers with concrete floors (AOR = 5.2, P = 0.004). The odds of detecting Campylobacter in milk were 17 times greater (AOR = 17, P = 0.007) in milk processing facilities that did not calibrate a pasteurizer on an annual basis. Finally, having a separate refrigerator for milk storage reduced the odds of detecting Campylobacter in retail (AOR = 0.29, P = 0.021).
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U2 - 10.1016/j.fm.2022.104145
DO - 10.1016/j.fm.2022.104145
M3 - Article
C2 - 36309427
AN - SCOPUS:85139738451
SN - 0740-0020
VL - 109
JO - Food Microbiology
JF - Food Microbiology
M1 - 104145
ER -