Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial

K. Woodson, D. Ratnasinghe, N. K. Bhat, C. Stewart, J. A. Tangrea, Terryl Johnson Hartman, R. Stolzenberg-Solomon, J. Virtamo, P. R. Taylor, D. Albanes

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Genetic susceptibility polymorphisms may be of substantial importance in the modulation of cancer risk. The prevalence for an array of polymorphic genes was determined in a cohort of male smokers who participated in a cancer prevention trial in Finland. A random sample of 120 individuals was selected from the trial cohort and the prevalence of variant alleles for nine genes was determined using a polymerase chain reaction-based approach. The prevalence values from this study were also compared with those of other populations derived from previous studies. Our results show that, with the exception of cytochrome P450-1A1 (CYP1A1) and cytochrome P450-2E1 (CYP2E1), all genes tested were sufficiently polymorphic to warrant an investigation of gene-environment studies. Most of the variant alleles, including alcohol dehydrogenase 3 (ADH3), glutathione-S-transferase (GSTM1), methionine synthase (MS), methylene tetrahydofolater reductase (MHTFR), CYP2E1 and CYP1A1, exhibited similar frequencies to other Caucasian populations. Interestingly, the prevalence of androgen receptor-CAG repeat (AR-CAG) and vitamin D receptor (VDR) polymorphisms differed significantly between the alpha-trocopherol, beta-carotene (ATBC) Study and other Caucasian populations. We present herein results from this survey and conclude that the ATBC study population in Finland is sufficiently heterogeneous to facilitate analysis of genetic polymorphisms and disease associations.

Original languageEnglish (US)
Pages (from-to)441-447
Number of pages7
JournalEuropean Journal of Cancer Prevention
Volume8
Issue number5
StatePublished - Dec 1 1999

Fingerprint

Cytochrome P-450 CYP2E1
DNA
beta Carotene
Finland
Cytochrome P-450 Enzyme System
Population
Genes
Neoplasms
Alleles
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase
Inborn Genetic Diseases
Calcitriol Receptors
Alcohol Dehydrogenase
Androgen Receptors
Genetic Polymorphisms
Genetic Predisposition to Disease
Glutathione Transferase
Oxidoreductases
Cross-Sectional Studies
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Woodson, K., Ratnasinghe, D., Bhat, N. K., Stewart, C., Tangrea, J. A., Hartman, T. J., ... Albanes, D. (1999). Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial. European Journal of Cancer Prevention, 8(5), 441-447.
Woodson, K. ; Ratnasinghe, D. ; Bhat, N. K. ; Stewart, C. ; Tangrea, J. A. ; Hartman, Terryl Johnson ; Stolzenberg-Solomon, R. ; Virtamo, J. ; Taylor, P. R. ; Albanes, D. / Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial. In: European Journal of Cancer Prevention. 1999 ; Vol. 8, No. 5. pp. 441-447.
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Woodson, K, Ratnasinghe, D, Bhat, NK, Stewart, C, Tangrea, JA, Hartman, TJ, Stolzenberg-Solomon, R, Virtamo, J, Taylor, PR & Albanes, D 1999, 'Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial', European Journal of Cancer Prevention, vol. 8, no. 5, pp. 441-447.

Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial. / Woodson, K.; Ratnasinghe, D.; Bhat, N. K.; Stewart, C.; Tangrea, J. A.; Hartman, Terryl Johnson; Stolzenberg-Solomon, R.; Virtamo, J.; Taylor, P. R.; Albanes, D.

In: European Journal of Cancer Prevention, Vol. 8, No. 5, 01.12.1999, p. 441-447.

Research output: Contribution to journalArticle

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T1 - Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial

AU - Woodson, K.

AU - Ratnasinghe, D.

AU - Bhat, N. K.

AU - Stewart, C.

AU - Tangrea, J. A.

AU - Hartman, Terryl Johnson

AU - Stolzenberg-Solomon, R.

AU - Virtamo, J.

AU - Taylor, P. R.

AU - Albanes, D.

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N2 - Genetic susceptibility polymorphisms may be of substantial importance in the modulation of cancer risk. The prevalence for an array of polymorphic genes was determined in a cohort of male smokers who participated in a cancer prevention trial in Finland. A random sample of 120 individuals was selected from the trial cohort and the prevalence of variant alleles for nine genes was determined using a polymerase chain reaction-based approach. The prevalence values from this study were also compared with those of other populations derived from previous studies. Our results show that, with the exception of cytochrome P450-1A1 (CYP1A1) and cytochrome P450-2E1 (CYP2E1), all genes tested were sufficiently polymorphic to warrant an investigation of gene-environment studies. Most of the variant alleles, including alcohol dehydrogenase 3 (ADH3), glutathione-S-transferase (GSTM1), methionine synthase (MS), methylene tetrahydofolater reductase (MHTFR), CYP2E1 and CYP1A1, exhibited similar frequencies to other Caucasian populations. Interestingly, the prevalence of androgen receptor-CAG repeat (AR-CAG) and vitamin D receptor (VDR) polymorphisms differed significantly between the alpha-trocopherol, beta-carotene (ATBC) Study and other Caucasian populations. We present herein results from this survey and conclude that the ATBC study population in Finland is sufficiently heterogeneous to facilitate analysis of genetic polymorphisms and disease associations.

AB - Genetic susceptibility polymorphisms may be of substantial importance in the modulation of cancer risk. The prevalence for an array of polymorphic genes was determined in a cohort of male smokers who participated in a cancer prevention trial in Finland. A random sample of 120 individuals was selected from the trial cohort and the prevalence of variant alleles for nine genes was determined using a polymerase chain reaction-based approach. The prevalence values from this study were also compared with those of other populations derived from previous studies. Our results show that, with the exception of cytochrome P450-1A1 (CYP1A1) and cytochrome P450-2E1 (CYP2E1), all genes tested were sufficiently polymorphic to warrant an investigation of gene-environment studies. Most of the variant alleles, including alcohol dehydrogenase 3 (ADH3), glutathione-S-transferase (GSTM1), methionine synthase (MS), methylene tetrahydofolater reductase (MHTFR), CYP2E1 and CYP1A1, exhibited similar frequencies to other Caucasian populations. Interestingly, the prevalence of androgen receptor-CAG repeat (AR-CAG) and vitamin D receptor (VDR) polymorphisms differed significantly between the alpha-trocopherol, beta-carotene (ATBC) Study and other Caucasian populations. We present herein results from this survey and conclude that the ATBC study population in Finland is sufficiently heterogeneous to facilitate analysis of genetic polymorphisms and disease associations.

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Woodson K, Ratnasinghe D, Bhat NK, Stewart C, Tangrea JA, Hartman TJ et al. Prevalence of disease-related DNA polymorphisms among participants in a large cancer prevention trial. European Journal of Cancer Prevention. 1999 Dec 1;8(5):441-447.