Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012

Zenglei Wang, Sony Shrestha, Xiaolian Li, Jun Miao, Lili Yuan, Mynthia Cabrera, Caitlin Grube, Zhaoqing Yang, Liwang Cui

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: The recent emergence and spread of artemisinin resistance in the Greater Mekong Subregion poses a great threat to malaria control and elimination. A K13-propeller gene (K13), PF3D7-1343700, has been associated lately with artemisinin resistance both in vitro and in vivo. This study aimed to investigate the K13 polymorphisms in Plasmodium falciparum parasites from the China-Myanmar border area where artemisinin use has the longest history. Methods: A total of 180 archived P. falciparum isolates containing 191 parasite clones, mainly collected in 2007-2012 from the China-Myanmar area, were used to obtain the full-length K13 gene sequences. Results: Seventeen point mutations were identified in 46.1% (88/191) parasite clones, of which seven were new. The F446I mutation predominated in 27.2% of the parasite clones. The C580Y mutation that is correlated with artemisinin resistance was detected at a low frequency of 1.6%. Collectively, 43.1% of the parasite clones contained point mutations in the kelch domain of the K13 gene. Moreover, there was a trend of increase in the frequency of parasites carrying kelch domain mutations through the years of sample collection. In addition, a microsatellite variation in the N-terminus of the K13 protein was found to have reached a high frequency (69.1%). Conclusions: This study documented the presence of mutations in the K13 gene in parasite populations from the China-Myanmar border. Mutations present in the kelch domain have become prevalent (>40%). A predominant mutation F446I and a prevalent microsatellite variation in the N-terminus were identified, but their importance in artemisinin resistance remains to be elucidated.

Original languageEnglish (US)
Article number168
JournalMalaria journal
Volume14
Issue number1
DOIs
StatePublished - Apr 18 2015

Fingerprint

Myanmar
Plasmodium falciparum
China
Parasites
Mutation
Clone Cells
Point Mutation
Microsatellite Repeats
Genes
Malaria
History
artemisinine

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Wang, Zenglei ; Shrestha, Sony ; Li, Xiaolian ; Miao, Jun ; Yuan, Lili ; Cabrera, Mynthia ; Grube, Caitlin ; Yang, Zhaoqing ; Cui, Liwang. / Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012. In: Malaria journal. 2015 ; Vol. 14, No. 1.
@article{f3a0ad876a064367ab3096db4e03374c,
title = "Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012",
abstract = "Background: The recent emergence and spread of artemisinin resistance in the Greater Mekong Subregion poses a great threat to malaria control and elimination. A K13-propeller gene (K13), PF3D7-1343700, has been associated lately with artemisinin resistance both in vitro and in vivo. This study aimed to investigate the K13 polymorphisms in Plasmodium falciparum parasites from the China-Myanmar border area where artemisinin use has the longest history. Methods: A total of 180 archived P. falciparum isolates containing 191 parasite clones, mainly collected in 2007-2012 from the China-Myanmar area, were used to obtain the full-length K13 gene sequences. Results: Seventeen point mutations were identified in 46.1{\%} (88/191) parasite clones, of which seven were new. The F446I mutation predominated in 27.2{\%} of the parasite clones. The C580Y mutation that is correlated with artemisinin resistance was detected at a low frequency of 1.6{\%}. Collectively, 43.1{\%} of the parasite clones contained point mutations in the kelch domain of the K13 gene. Moreover, there was a trend of increase in the frequency of parasites carrying kelch domain mutations through the years of sample collection. In addition, a microsatellite variation in the N-terminus of the K13 protein was found to have reached a high frequency (69.1{\%}). Conclusions: This study documented the presence of mutations in the K13 gene in parasite populations from the China-Myanmar border. Mutations present in the kelch domain have become prevalent (>40{\%}). A predominant mutation F446I and a prevalent microsatellite variation in the N-terminus were identified, but their importance in artemisinin resistance remains to be elucidated.",
author = "Zenglei Wang and Sony Shrestha and Xiaolian Li and Jun Miao and Lili Yuan and Mynthia Cabrera and Caitlin Grube and Zhaoqing Yang and Liwang Cui",
year = "2015",
month = "4",
day = "18",
doi = "10.1186/s12936-015-0672-9",
language = "English (US)",
volume = "14",
journal = "Malaria Journal",
issn = "1475-2875",
publisher = "BioMed Central",
number = "1",

}

Wang, Z, Shrestha, S, Li, X, Miao, J, Yuan, L, Cabrera, M, Grube, C, Yang, Z & Cui, L 2015, 'Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012', Malaria journal, vol. 14, no. 1, 168. https://doi.org/10.1186/s12936-015-0672-9

Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012. / Wang, Zenglei; Shrestha, Sony; Li, Xiaolian; Miao, Jun; Yuan, Lili; Cabrera, Mynthia; Grube, Caitlin; Yang, Zhaoqing; Cui, Liwang.

In: Malaria journal, Vol. 14, No. 1, 168, 18.04.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevalence of K13-propeller polymorphisms in Plasmodium falciparum from China-Myanmar border in 2007-2012

AU - Wang, Zenglei

AU - Shrestha, Sony

AU - Li, Xiaolian

AU - Miao, Jun

AU - Yuan, Lili

AU - Cabrera, Mynthia

AU - Grube, Caitlin

AU - Yang, Zhaoqing

AU - Cui, Liwang

PY - 2015/4/18

Y1 - 2015/4/18

N2 - Background: The recent emergence and spread of artemisinin resistance in the Greater Mekong Subregion poses a great threat to malaria control and elimination. A K13-propeller gene (K13), PF3D7-1343700, has been associated lately with artemisinin resistance both in vitro and in vivo. This study aimed to investigate the K13 polymorphisms in Plasmodium falciparum parasites from the China-Myanmar border area where artemisinin use has the longest history. Methods: A total of 180 archived P. falciparum isolates containing 191 parasite clones, mainly collected in 2007-2012 from the China-Myanmar area, were used to obtain the full-length K13 gene sequences. Results: Seventeen point mutations were identified in 46.1% (88/191) parasite clones, of which seven were new. The F446I mutation predominated in 27.2% of the parasite clones. The C580Y mutation that is correlated with artemisinin resistance was detected at a low frequency of 1.6%. Collectively, 43.1% of the parasite clones contained point mutations in the kelch domain of the K13 gene. Moreover, there was a trend of increase in the frequency of parasites carrying kelch domain mutations through the years of sample collection. In addition, a microsatellite variation in the N-terminus of the K13 protein was found to have reached a high frequency (69.1%). Conclusions: This study documented the presence of mutations in the K13 gene in parasite populations from the China-Myanmar border. Mutations present in the kelch domain have become prevalent (>40%). A predominant mutation F446I and a prevalent microsatellite variation in the N-terminus were identified, but their importance in artemisinin resistance remains to be elucidated.

AB - Background: The recent emergence and spread of artemisinin resistance in the Greater Mekong Subregion poses a great threat to malaria control and elimination. A K13-propeller gene (K13), PF3D7-1343700, has been associated lately with artemisinin resistance both in vitro and in vivo. This study aimed to investigate the K13 polymorphisms in Plasmodium falciparum parasites from the China-Myanmar border area where artemisinin use has the longest history. Methods: A total of 180 archived P. falciparum isolates containing 191 parasite clones, mainly collected in 2007-2012 from the China-Myanmar area, were used to obtain the full-length K13 gene sequences. Results: Seventeen point mutations were identified in 46.1% (88/191) parasite clones, of which seven were new. The F446I mutation predominated in 27.2% of the parasite clones. The C580Y mutation that is correlated with artemisinin resistance was detected at a low frequency of 1.6%. Collectively, 43.1% of the parasite clones contained point mutations in the kelch domain of the K13 gene. Moreover, there was a trend of increase in the frequency of parasites carrying kelch domain mutations through the years of sample collection. In addition, a microsatellite variation in the N-terminus of the K13 protein was found to have reached a high frequency (69.1%). Conclusions: This study documented the presence of mutations in the K13 gene in parasite populations from the China-Myanmar border. Mutations present in the kelch domain have become prevalent (>40%). A predominant mutation F446I and a prevalent microsatellite variation in the N-terminus were identified, but their importance in artemisinin resistance remains to be elucidated.

UR - http://www.scopus.com/inward/record.url?scp=84928014326&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928014326&partnerID=8YFLogxK

U2 - 10.1186/s12936-015-0672-9

DO - 10.1186/s12936-015-0672-9

M3 - Article

VL - 14

JO - Malaria Journal

JF - Malaria Journal

SN - 1475-2875

IS - 1

M1 - 168

ER -