Prevention of exuberant granulation tissue and neovascularization in the rat cornea by naltrexone

Ian S. Zagon, Matthew S. Klocek, James W. Griffith, Joseph W. Sassani, András M. Komáromy, Patricia J. McLaughlin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To determine whether topical application of naltrexone prevents exuberant granulation tissue formation with neovascularization in diabetic rat corneas. Methods: Diabetes was induced with streptozotocin. A 5-mm corneal abrasion at 9 or 11 weeks was treated topically for 7 days (4 times daily) with naltrexone or a sterile vehicle. Results: Within 2 to 5 days after reepithelialization, diabetic rats given the sterile vehicle had a 41% incidence of corneal lesions represented by exuberant granulation tissue with corneal neovascularization extending from the limbus. These lesions exhibited edema, cellular and vascular inflammation, and disruption of stromal lamella by fibrovascular tissue and calcium mineralization, but infection was not detected. No corneal lesions were recorded in the diabetic group treated with naltrexone or the control group given the sterile vehicle. Diabetic rats with corneal lesions given the sterile vehicle reepithelialized more slowly than diabetic rats given the sterile vehicle without such lesions, but no difference in blood glucose levels were noted. Conclusions: Using a minimally invasive model in diabetic rats, topical naltrexone normalizes corneal wound healing and prevents neovascularization. Clinical Relevance: Direct application of naltrexone may serve as an important strategy for facilitating corneal healing and inhibiting corneal neovascularization.

Original languageEnglish (US)
Pages (from-to)501-506
Number of pages6
JournalArchives of Ophthalmology
Volume126
Issue number4
DOIs
StatePublished - Apr 1 2008

Fingerprint

Naltrexone
Granulation Tissue
Cornea
Corneal Neovascularization
Streptozocin
Wound Healing
Blood Vessels
Blood Glucose
Edema
Inflammation
Calcium
Control Groups
Incidence
Infection

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

@article{2a13a2cffc404c72a109672462dd512c,
title = "Prevention of exuberant granulation tissue and neovascularization in the rat cornea by naltrexone",
abstract = "Objective: To determine whether topical application of naltrexone prevents exuberant granulation tissue formation with neovascularization in diabetic rat corneas. Methods: Diabetes was induced with streptozotocin. A 5-mm corneal abrasion at 9 or 11 weeks was treated topically for 7 days (4 times daily) with naltrexone or a sterile vehicle. Results: Within 2 to 5 days after reepithelialization, diabetic rats given the sterile vehicle had a 41{\%} incidence of corneal lesions represented by exuberant granulation tissue with corneal neovascularization extending from the limbus. These lesions exhibited edema, cellular and vascular inflammation, and disruption of stromal lamella by fibrovascular tissue and calcium mineralization, but infection was not detected. No corneal lesions were recorded in the diabetic group treated with naltrexone or the control group given the sterile vehicle. Diabetic rats with corneal lesions given the sterile vehicle reepithelialized more slowly than diabetic rats given the sterile vehicle without such lesions, but no difference in blood glucose levels were noted. Conclusions: Using a minimally invasive model in diabetic rats, topical naltrexone normalizes corneal wound healing and prevents neovascularization. Clinical Relevance: Direct application of naltrexone may serve as an important strategy for facilitating corneal healing and inhibiting corneal neovascularization.",
author = "Zagon, {Ian S.} and Klocek, {Matthew S.} and Griffith, {James W.} and Sassani, {Joseph W.} and Kom{\'a}romy, {Andr{\'a}s M.} and McLaughlin, {Patricia J.}",
year = "2008",
month = "4",
day = "1",
doi = "10.1001/archopht.126.4.501",
language = "English (US)",
volume = "126",
pages = "501--506",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "4",

}

Prevention of exuberant granulation tissue and neovascularization in the rat cornea by naltrexone. / Zagon, Ian S.; Klocek, Matthew S.; Griffith, James W.; Sassani, Joseph W.; Komáromy, András M.; McLaughlin, Patricia J.

In: Archives of Ophthalmology, Vol. 126, No. 4, 01.04.2008, p. 501-506.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevention of exuberant granulation tissue and neovascularization in the rat cornea by naltrexone

AU - Zagon, Ian S.

AU - Klocek, Matthew S.

AU - Griffith, James W.

AU - Sassani, Joseph W.

AU - Komáromy, András M.

AU - McLaughlin, Patricia J.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Objective: To determine whether topical application of naltrexone prevents exuberant granulation tissue formation with neovascularization in diabetic rat corneas. Methods: Diabetes was induced with streptozotocin. A 5-mm corneal abrasion at 9 or 11 weeks was treated topically for 7 days (4 times daily) with naltrexone or a sterile vehicle. Results: Within 2 to 5 days after reepithelialization, diabetic rats given the sterile vehicle had a 41% incidence of corneal lesions represented by exuberant granulation tissue with corneal neovascularization extending from the limbus. These lesions exhibited edema, cellular and vascular inflammation, and disruption of stromal lamella by fibrovascular tissue and calcium mineralization, but infection was not detected. No corneal lesions were recorded in the diabetic group treated with naltrexone or the control group given the sterile vehicle. Diabetic rats with corneal lesions given the sterile vehicle reepithelialized more slowly than diabetic rats given the sterile vehicle without such lesions, but no difference in blood glucose levels were noted. Conclusions: Using a minimally invasive model in diabetic rats, topical naltrexone normalizes corneal wound healing and prevents neovascularization. Clinical Relevance: Direct application of naltrexone may serve as an important strategy for facilitating corneal healing and inhibiting corneal neovascularization.

AB - Objective: To determine whether topical application of naltrexone prevents exuberant granulation tissue formation with neovascularization in diabetic rat corneas. Methods: Diabetes was induced with streptozotocin. A 5-mm corneal abrasion at 9 or 11 weeks was treated topically for 7 days (4 times daily) with naltrexone or a sterile vehicle. Results: Within 2 to 5 days after reepithelialization, diabetic rats given the sterile vehicle had a 41% incidence of corneal lesions represented by exuberant granulation tissue with corneal neovascularization extending from the limbus. These lesions exhibited edema, cellular and vascular inflammation, and disruption of stromal lamella by fibrovascular tissue and calcium mineralization, but infection was not detected. No corneal lesions were recorded in the diabetic group treated with naltrexone or the control group given the sterile vehicle. Diabetic rats with corneal lesions given the sterile vehicle reepithelialized more slowly than diabetic rats given the sterile vehicle without such lesions, but no difference in blood glucose levels were noted. Conclusions: Using a minimally invasive model in diabetic rats, topical naltrexone normalizes corneal wound healing and prevents neovascularization. Clinical Relevance: Direct application of naltrexone may serve as an important strategy for facilitating corneal healing and inhibiting corneal neovascularization.

UR - http://www.scopus.com/inward/record.url?scp=42249089840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42249089840&partnerID=8YFLogxK

U2 - 10.1001/archopht.126.4.501

DO - 10.1001/archopht.126.4.501

M3 - Article

C2 - 18413519

AN - SCOPUS:42249089840

VL - 126

SP - 501

EP - 506

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 4

ER -