Primary cilia as sensors of mechanical loading

S. Temiyasathit, W. J. Tang, Charles T. Anderson, T. P. Stearns, C. R. Jacobs

Research output: Contribution to journalArticlepeer-review

Abstract

Transgenic mice with bone specific knockout of Kif3A are used to study the role of primary cilia in the response of bone to mechanical loading. Beginning with day 1, loading consists of three consecutive daily ulna loading sessions of 1 minute exposure to 2.5N sinusoidal loading for 120 cycles. The unloaded contralateral ulna serves as a control. Calcein labels are administered by subcutaneous injection on days 5 and 9. After euthanization on day 15, the ulnae are embedded in methyl methacrylate and sections are prepared from the mid-diaphysis. Dynamic histomorphometric analysis is conducted on sections to determine the standard measure of bone formation rate at the periosteal surface of loaded and unloaded ulnae of each mouse. Observations focused on total perimeter, single label perimeter, double label perimeter and double label area.

Original languageEnglish (US)
Pages (from-to)215-216
Number of pages2
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number4
StatePublished - Dec 1 2006

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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