Primary cilia as sensors of mechanical loading

S. Temiyasathit, W. J. Tang, Charles T. Anderson, T. P. Stearns, C. R. Jacobs

Research output: Contribution to journalArticle

Abstract

Transgenic mice with bone specific knockout of Kif3A are used to study the role of primary cilia in the response of bone to mechanical loading. Beginning with day 1, loading consists of three consecutive daily ulna loading sessions of 1 minute exposure to 2.5N sinusoidal loading for 120 cycles. The unloaded contralateral ulna serves as a control. Calcein labels are administered by subcutaneous injection on days 5 and 9. After euthanization on day 15, the ulnae are embedded in methyl methacrylate and sections are prepared from the mid-diaphysis. Dynamic histomorphometric analysis is conducted on sections to determine the standard measure of bone formation rate at the periosteal surface of loaded and unloaded ulnae of each mouse. Observations focused on total perimeter, single label perimeter, double label perimeter and double label area.

Original languageEnglish (US)
Pages (from-to)215-216
Number of pages2
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number4
StatePublished - Dec 1 2006

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Ulna
Cilia
Bone and Bones
Diaphyses
Methacrylates
Subcutaneous Injections
Osteogenesis
Transgenic Mice

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Temiyasathit, S., Tang, W. J., Anderson, C. T., Stearns, T. P., & Jacobs, C. R. (2006). Primary cilia as sensors of mechanical loading. MCB Molecular and Cellular Biomechanics, 3(4), 215-216.
Temiyasathit, S. ; Tang, W. J. ; Anderson, Charles T. ; Stearns, T. P. ; Jacobs, C. R. / Primary cilia as sensors of mechanical loading. In: MCB Molecular and Cellular Biomechanics. 2006 ; Vol. 3, No. 4. pp. 215-216.
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Temiyasathit, S, Tang, WJ, Anderson, CT, Stearns, TP & Jacobs, CR 2006, 'Primary cilia as sensors of mechanical loading', MCB Molecular and Cellular Biomechanics, vol. 3, no. 4, pp. 215-216.

Primary cilia as sensors of mechanical loading. / Temiyasathit, S.; Tang, W. J.; Anderson, Charles T.; Stearns, T. P.; Jacobs, C. R.

In: MCB Molecular and Cellular Biomechanics, Vol. 3, No. 4, 01.12.2006, p. 215-216.

Research output: Contribution to journalArticle

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Temiyasathit S, Tang WJ, Anderson CT, Stearns TP, Jacobs CR. Primary cilia as sensors of mechanical loading. MCB Molecular and Cellular Biomechanics. 2006 Dec 1;3(4):215-216.