Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts

A. M.D. Malone, Charles T. Anderson, P. Tummala, T. Stearns, C. R. Jacobs

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

Original languageEnglish (US)
Pages (from-to)207-208
Number of pages2
JournalMCB Molecular and Cellular Biomechanics
Volume3
Issue number4
StatePublished - Dec 1 2006

Fingerprint

Cilia
Osteoblasts
Dinoprostone
Calcium
Bone and Bones
Tubulin
Enzyme-Linked Immunosorbent Assay
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Malone, A. M. D., Anderson, C. T., Tummala, P., Stearns, T., & Jacobs, C. R. (2006). Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts. MCB Molecular and Cellular Biomechanics, 3(4), 207-208.
Malone, A. M.D. ; Anderson, Charles T. ; Tummala, P. ; Stearns, T. ; Jacobs, C. R. / Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts. In: MCB Molecular and Cellular Biomechanics. 2006 ; Vol. 3, No. 4. pp. 207-208.
@article{ad24d32a92f2415dae7efd3216d497b1,
title = "Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts",
abstract = "To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.",
author = "Malone, {A. M.D.} and Anderson, {Charles T.} and P. Tummala and T. Stearns and Jacobs, {C. R.}",
year = "2006",
month = "12",
day = "1",
language = "English (US)",
volume = "3",
pages = "207--208",
journal = "MCB Molecular and Cellular Biomechanics",
issn = "1556-5297",
publisher = "Tech Science Press",
number = "4",

}

Malone, AMD, Anderson, CT, Tummala, P, Stearns, T & Jacobs, CR 2006, 'Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts', MCB Molecular and Cellular Biomechanics, vol. 3, no. 4, pp. 207-208.

Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts. / Malone, A. M.D.; Anderson, Charles T.; Tummala, P.; Stearns, T.; Jacobs, C. R.

In: MCB Molecular and Cellular Biomechanics, Vol. 3, No. 4, 01.12.2006, p. 207-208.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Primary cilia mediate PGE2 release in MC3T3-E1 osteoblasts

AU - Malone, A. M.D.

AU - Anderson, Charles T.

AU - Tummala, P.

AU - Stearns, T.

AU - Jacobs, C. R.

PY - 2006/12/1

Y1 - 2006/12/1

N2 - To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

AB - To determine whether primary cilia act as mechanosensors in bone, intracellular calcium release and prostaglandin E2 release were examined. The existence of primary cilia in bone tissue was confirmed using immunoflourescent detection of acetylated α-tubulin. PGE2 was quantifued using a commercial ELISA kiet and normalized to total protein. Real-time intracellular levels of calcium were quantified.

UR - http://www.scopus.com/inward/record.url?scp=33846458329&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846458329&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33846458329

VL - 3

SP - 207

EP - 208

JO - MCB Molecular and Cellular Biomechanics

JF - MCB Molecular and Cellular Biomechanics

SN - 1556-5297

IS - 4

ER -