7 Citations (Scopus)

Abstract

Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities comparable or better than antibodies. The SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere (with false positives and negatives) with the selection process. Here we present a primer-free protocol using a random DNA library.

Original languageEnglish (US)
Pages (from-to)369-385
Number of pages17
JournalMethods in molecular biology (Clifton, N.J.)
Volume629
DOIs
StatePublished - Jan 1 2010

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SELEX Aptamer Technique
Gene Library
Libraries
Oligonucleotides
Regeneration
Ligands
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

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author = "Weihua Pan and Gary Clawson",
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Primer-free aptamer selection using a random DNA library. / Pan, Weihua; Clawson, Gary.

In: Methods in molecular biology (Clifton, N.J.), Vol. 629, 01.01.2010, p. 369-385.

Research output: Contribution to journalArticle

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T1 - Primer-free aptamer selection using a random DNA library.

AU - Pan, Weihua

AU - Clawson, Gary

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Aptamers are high-affinity oligonucleotides which can be selected from large random libraries by systematic evolution of ligands by exponential enrichment (SELEX) protocols, with affinities and specificities comparable or better than antibodies. The SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere (with false positives and negatives) with the selection process. Here we present a primer-free protocol using a random DNA library.

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U2 - 10.1007/978-1-60761-657-3_24

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SP - 369

EP - 385

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

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