Principles and applications of polymerase chain reaction: Basic science for the practicing physician

Faoud Ishmael, Cristiana Stellato

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To review the technology of polymerase chain reaction (PCR) and its use in diagnostic medicine and biomedical research. Data Sources: A literature review was performed using the PubMed database for English-language articles published between January 1, 1985, and November 31, 2007, using the following keywords: polymerase chain reaction, real-time polymerase chain reaction, and applications of real-time polymerase chain reaction. Study Selection: Select review articles and primary articles relevant to medical applications of PCR were chosen. Results: As a revolutionary technique, PCR provides a rapid and accurate means of amplifying DNA. It has enabled the cloning and manipulation of genes for biomedical research and has facilitated the diagnosis of genetic, infectious, and oncologic diseases. The use of reverse transcriptases to evaluate RNA levels and the extension of PCR technology to quantify DNA amplification in real time has brought major advances to the application of PCR. By allowing the determination and quantification of changes in gene expression, these techniques have provided a greater understanding of disease processes and now serve as a foundation for diagnostics and basic science research. Conclusions: Both PCR and real-time PCR have dramatically changed the field of basic science and diagnostic medicine. These technologies are now a well-established, indispensable part of research and clinical laboratories.

Original languageEnglish (US)
Pages (from-to)437-443
Number of pages7
JournalAnnals of Allergy, Asthma and Immunology
Volume101
Issue number4
DOIs
StatePublished - Jan 1 2008

Fingerprint

Physicians
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Technology
Biomedical Research
Medicine
Information Storage and Retrieval
RNA-Directed DNA Polymerase
DNA
Research
PubMed
Communicable Diseases
Organism Cloning
Language
Databases
RNA
Gene Expression
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Cite this

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Principles and applications of polymerase chain reaction : Basic science for the practicing physician. / Ishmael, Faoud; Stellato, Cristiana.

In: Annals of Allergy, Asthma and Immunology, Vol. 101, No. 4, 01.01.2008, p. 437-443.

Research output: Contribution to journalArticle

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