PRK1, a receptor-like kinase of Petunia inflate, is essential for postmeiotic development of pollen

Hyun Sook Lee, Balasulojini Karunanandaa, Andrew McCubbin, Simon Gilroy, Teh-hui Kao

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

A pollen-expressed gene of Petunia inflate that encodes a receptor-like kinase named PRK1 was previously identified. The extracellular domain of PRK1 contains leucine-rich repeats which have been implicated in protein-protein interactions, and the cytoplasmic domain was found to autophosphorylate on serine and possibly tyrosine. To investigate the function of PRK1 in pollen development, P. inflate plants were transformed with a construct containing the promoter of a pollen-expressed gene of tomato, LAT52, fused to an antisense PRK1 cDNA corresponding to part of the extracellular domain of PRK1. Three transgenic plants were found to each produce approximately equal amounts of normal and aborted pollen. Analysis of the inheritance of the transgene inserts in two of the transgenic plants, ASRK-13 and ASRK-20, to their progeny revealed that certain transgene inserts co-segregated with the pollen abortion phenotype. Microscopic examination of the aborted pollen grains showed that their outer wall, the exine, was essentially normal, but that their cytoplasm contained only starch-like granules. Staining of the nuclei of the microspores at different stages of anther development revealed that the microspores of the transgenic plants developed normally until the uninucleate stage. However, in subsequent stages half of the microspores completed mitosis and developed into normal binucleate pollen, but the other half initially remained uninucleate and subsequently lost their nuclei. Analysis of the amounts of PRKI mRIMA and the antisense PRK1 transcript suggested that the pollen abortion phenotype most likely resulted from downregulation of the PRK1 gene by the antisense PRK1 transgene. These results suggest that PRK1 plays an essential role in a signal transduction pathway that mediates postmeiotic development of microspores.

Original languageEnglish (US)
Pages (from-to)613-624
Number of pages12
JournalPlant Journal
Volume9
Issue number5
DOIs
StatePublished - Jan 1 1996

Fingerprint

Petunia
Pollen
phosphotransferases (kinases)
Phosphotransferases
pollen
microspores
receptors
transgenes
transgenic plants
Genetically Modified Plants
abortion (plants)
Transgenes
phenotype
genes
exine
protein-protein interactions
Protein Interaction Domains and Motifs
Genes
Phenotype
anthers

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Lee, Hyun Sook ; Karunanandaa, Balasulojini ; McCubbin, Andrew ; Gilroy, Simon ; Kao, Teh-hui. / PRK1, a receptor-like kinase of Petunia inflate, is essential for postmeiotic development of pollen. In: Plant Journal. 1996 ; Vol. 9, No. 5. pp. 613-624.
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PRK1, a receptor-like kinase of Petunia inflate, is essential for postmeiotic development of pollen. / Lee, Hyun Sook; Karunanandaa, Balasulojini; McCubbin, Andrew; Gilroy, Simon; Kao, Teh-hui.

In: Plant Journal, Vol. 9, No. 5, 01.01.1996, p. 613-624.

Research output: Contribution to journalArticle

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