Probing cell-cell communication with microfluidic devices

Feng Guo, Jarrod B. French, Peng Li, Hong Zhao, Chung Yu Chan, James R. Fick, Stephen J. Benkovic, Tony Jun Huang

Research output: Contribution to journalReview article

43 Citations (Scopus)

Abstract

Intercellular communication is a mechanism that regulates critical events during embryogenesis and coordinates signalling within differentiated tissues, such as the nervous and cardiovascular systems. To perform specialized activities, these tissues utilize the rapid exchange of signals among networks that, while are composed of different cell types, are nevertheless functionally coupled. Errors in cellular communication can lead to varied deleterious effects such as degenerative and autoimmune diseases. However, the intercellular communication network is extremely complex in multicellular organisms making isolation of the functional unit and study of basic mechanisms technically challenging. New experimental methods to examine mechanisms of intercellular communication among cultured cells could provide insight into physiological and pathological processes alike. Recent developments in microfluidic technology allow miniaturized and integrated devices to perform intercellular communication experiments on-chip. Microfluidics have many advantages, including the ability to replicate in vitro the chemical, mechanical, and physical cellular microenvironment of tissues with precise spatial and temporal control combined with dynamic characterization, high throughput, scalability and reproducibility. In this Focus article, we highlight some of the recent work and advances in the application of microfluidics to the study of mammalian intercellular communication with particular emphasis on cell contact and soluble factor mediated communication. In addition, we provide some insights into likely direction of the future developments in this field.

Original languageEnglish (US)
Pages (from-to)3152-3162
Number of pages11
JournalLab on a Chip
Volume13
Issue number16
DOIs
StatePublished - Aug 21 2013

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
Cell Communication
Communication
Physiological Phenomena
Cellular Microenvironment
Tissue
Pathologic Processes
Cardiovascular System
Nervous System
Autoimmune Diseases
Embryonic Development
Cultured Cells
Cardiovascular system
Cellular radio systems
Technology
Neurology
Equipment and Supplies
Telecommunication networks
Scalability

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Guo, F., French, J. B., Li, P., Zhao, H., Chan, C. Y., Fick, J. R., ... Huang, T. J. (2013). Probing cell-cell communication with microfluidic devices. Lab on a Chip, 13(16), 3152-3162. https://doi.org/10.1039/c3lc90067c
Guo, Feng ; French, Jarrod B. ; Li, Peng ; Zhao, Hong ; Chan, Chung Yu ; Fick, James R. ; Benkovic, Stephen J. ; Huang, Tony Jun. / Probing cell-cell communication with microfluidic devices. In: Lab on a Chip. 2013 ; Vol. 13, No. 16. pp. 3152-3162.
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Guo, F, French, JB, Li, P, Zhao, H, Chan, CY, Fick, JR, Benkovic, SJ & Huang, TJ 2013, 'Probing cell-cell communication with microfluidic devices', Lab on a Chip, vol. 13, no. 16, pp. 3152-3162. https://doi.org/10.1039/c3lc90067c

Probing cell-cell communication with microfluidic devices. / Guo, Feng; French, Jarrod B.; Li, Peng; Zhao, Hong; Chan, Chung Yu; Fick, James R.; Benkovic, Stephen J.; Huang, Tony Jun.

In: Lab on a Chip, Vol. 13, No. 16, 21.08.2013, p. 3152-3162.

Research output: Contribution to journalReview article

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Guo F, French JB, Li P, Zhao H, Chan CY, Fick JR et al. Probing cell-cell communication with microfluidic devices. Lab on a Chip. 2013 Aug 21;13(16):3152-3162. https://doi.org/10.1039/c3lc90067c