Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement

Yongliang Yang, Nima Jamilpour, Baoyin Yao, Zachary S. Dean, Reza Riahi, Pak Kin Wong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

When blood vessels are injured, leader cells emerge in the endothelium to heal the wound and restore the vasculature integrity. The characteristics of leader cells during endothelial collective migration under diverse physiological conditions, however, are poorly understood. Here we investigate the regulation and function of endothelial leader cells by plasma lithography geometric confinement generated. Endothelial leader cells display an aggressive phenotype, connect to follower cells via peripheral actin cables and discontinuous adherens junctions, and lead migrating clusters near the leading edge. Time-lapse microscopy, immunostaining, and particle image velocimetry reveal that the density of leader cells and the speed of migrating clusters are tightly regulated in a wide range of geometric patterns. By challenging the cells with converging, diverging and competing patterns, we show that the density of leader cells correlates with the size and coherence of the migrating clusters. Collectively, our data provide evidence that leader cells control endothelial collective migration by regualting the migrating clusters.

Original languageEnglish (US)
Article number22707
JournalScientific reports
Volume6
DOIs
StatePublished - Mar 3 2016

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Endothelial Cells
Cell Count
Adherens Junctions
Rheology
Endothelium
Blood Vessels
Actins
Microscopy
Phenotype
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • General

Cite this

Yang, Yongliang ; Jamilpour, Nima ; Yao, Baoyin ; Dean, Zachary S. ; Riahi, Reza ; Wong, Pak Kin. / Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement. In: Scientific reports. 2016 ; Vol. 6.
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Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement. / Yang, Yongliang; Jamilpour, Nima; Yao, Baoyin; Dean, Zachary S.; Riahi, Reza; Wong, Pak Kin.

In: Scientific reports, Vol. 6, 22707, 03.03.2016.

Research output: Contribution to journalArticle

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