Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors

Chunfeng Zhang, Justin Ingram, Steven Schiff, Jian Xu, Min Xiao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

We developed ratiometric optical oxygen sensors to probe the oxygen consumption during epileptic events in rat brain slices. The oxygen sensors consist of the sensing part of phosphorescence dyes (Platinum (II) octaethylporphine ketone) and reference part of nanocystal quantum dots (NQDs) embedded in polymer blends, with pre-designed excitation through fluorescence resonance energy transfer (FRET) from NQDs to the oxygen sensitive dyes (OSDs). The ratiometric FRET sensors with fast temporal response and excellent bio-compatibility are suitable for real time quantitative dissolved oxygen (D.O.) probes in biological microenvironment. Coating the sensors onto the micro-pipettes, we performed simultaneous oxygen probes at pyramidal and oriens layers in rat hippocampal CA1. Different spatiotemporal patterns with maximum D.O. decreases of 9.9±1.1 mg/L and 4.9±0.7 mg/L during seizure events were observed in pyramidal and oriens layers, respectively.

Original languageEnglish (US)
Title of host publicationQuantum Dots and Nanostructures
Subtitle of host publicationSynthesis, Characterization, and Modeling VIII
Volume7947
DOIs
StatePublished - Apr 27 2011
EventQuantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII - San Francisco, CA, United States
Duration: Jan 25 2011Jan 26 2011

Other

OtherQuantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII
CountryUnited States
CitySan Francisco, CA
Period1/25/111/26/11

Fingerprint

oxygen consumption
Oxygen Consumption
Energy Transfer
resonance fluorescence
Slice
Fluorescence
brain
Oxygen
Oxygen sensors
Brain
energy transfer
Dissolved oxygen
Sensor
Semiconductor quantum dots
Rats
sensors
Sensors
oxygen
Coloring Agents
Dyes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Zhang, C., Ingram, J., Schiff, S., Xu, J., & Xiao, M. (2011). Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. In Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII (Vol. 7947). [79470B] https://doi.org/10.1117/12.876341
Zhang, Chunfeng ; Ingram, Justin ; Schiff, Steven ; Xu, Jian ; Xiao, Min. / Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII. Vol. 7947 2011.
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Zhang, C, Ingram, J, Schiff, S, Xu, J & Xiao, M 2011, Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. in Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII. vol. 7947, 79470B, Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII, San Francisco, CA, United States, 1/25/11. https://doi.org/10.1117/12.876341

Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. / Zhang, Chunfeng; Ingram, Justin; Schiff, Steven; Xu, Jian; Xiao, Min.

Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII. Vol. 7947 2011. 79470B.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Zhang C, Ingram J, Schiff S, Xu J, Xiao M. Probing oxygen consumption in epileptic brain slices with QDs-based FRET sensors. In Quantum Dots and Nanostructures: Synthesis, Characterization, and Modeling VIII. Vol. 7947. 2011. 79470B https://doi.org/10.1117/12.876341