Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity

R. Rajesh, Raghavendra Gowda, A. Nataraju, B. L. Dhananjaya, K. Kemparaju, B. S. Vishwanath

Research output: Contribution to journalArticle

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Abstract

The latex of Calotropis gigantea is a rich source of useful components that has medicinal properties and one of the main applications is in controlling bleeding. The crude latex extract contained many proteins, which are highly basic in nature and exhibited strong proteolytic activity. The crude extract hydrolyses casein, human fibrinogen and crude fibrin clot in a dose-dependent manner. The hydrolyzing activity was completely inhibited by IAA indicating they belong to the super family, cysteine proteases. Crude extract hydrolyses Aα, Bβ and γ subunits of fibrinogen. Among all the subunits the preferential subunit to get hydrolyzed was Aα followed by Bβ and γ subunit is highly resistant and hydrolyzed at higher protein concentration or over a prolonged incubation time. The crude extract hydrolysis crude fibrin clot strongly compared to trypsin and papain. Pharmacologically the crude extract is hemorrhagic and induces skin hemorrhage at >75 μg and reduces the coagulation time of citrated plasma from 150 to 47 s and promotes blood coagulation. Procoagulation and blood clot hydrolysis are important in wound healing process. This is due to unique cysteine proteases of plant latex and is responsible for the pharmacological actions observed in folk medicine.

Original languageEnglish (US)
Pages (from-to)84-92
Number of pages9
JournalToxicon
Volume46
Issue number1
DOIs
StatePublished - Jul 1 2005

Fingerprint

Calotropis
Latex
Fibrin
Complex Mixtures
Hydrolysis
Cysteine Proteases
Coagulation
Fibrinogen
Blood
Papain
Hemorrhage
Caseins
Trypsin
Medicine
Traditional Medicine
Blood Coagulation
Skin
Proteins
Wound Healing
Plasmas

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Rajesh, R., Gowda, R., Nataraju, A., Dhananjaya, B. L., Kemparaju, K., & Vishwanath, B. S. (2005). Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity. Toxicon, 46(1), 84-92. https://doi.org/10.1016/j.toxicon.2005.03.012
Rajesh, R. ; Gowda, Raghavendra ; Nataraju, A. ; Dhananjaya, B. L. ; Kemparaju, K. ; Vishwanath, B. S. / Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity. In: Toxicon. 2005 ; Vol. 46, No. 1. pp. 84-92.
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Rajesh, R, Gowda, R, Nataraju, A, Dhananjaya, BL, Kemparaju, K & Vishwanath, BS 2005, 'Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity', Toxicon, vol. 46, no. 1, pp. 84-92. https://doi.org/10.1016/j.toxicon.2005.03.012

Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity. / Rajesh, R.; Gowda, Raghavendra; Nataraju, A.; Dhananjaya, B. L.; Kemparaju, K.; Vishwanath, B. S.

In: Toxicon, Vol. 46, No. 1, 01.07.2005, p. 84-92.

Research output: Contribution to journalArticle

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Rajesh R, Gowda R, Nataraju A, Dhananjaya BL, Kemparaju K, Vishwanath BS. Procoagulant activity of Calotropis gigantea latex associated with fibrin(ogen)olytic activity. Toxicon. 2005 Jul 1;46(1):84-92. https://doi.org/10.1016/j.toxicon.2005.03.012