Production and characterization of a novel HPV anti-L2 monoclonal antibody panel

S. M. Bywaters, S. A. Brendle, J. Biryukov, J. W. Wang, J. Walston, J. Milici, R. B. Roden, Craig Meyers, Neil Christensen

Research output: Contribution to journalArticle

Abstract

The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-terminus. As a means to better define the virus capsid and better assess the localization and exposure of L2 epitopes in the context of assembled HPV, we have developed a panel of 30 monoclonal antibodies (mAbs) which target the N-terminus of L2 amino acids 11–200, previously defined as a broadly protective immunogen. Select mAbs were processed with enzymes and anti-L2 Fabs were generated. These new mAb/Fab probes will be beneficial in future studies to unravel the placement of L2 and to help better define the role of L2 in the HPV lifecycle and the nature of the broadly protective epitopes.

Original languageEnglish (US)
Pages (from-to)106-113
Number of pages8
JournalVirology
Volume524
DOIs
StatePublished - Nov 1 2018

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Epitopes
Monoclonal Antibodies
Capsid Proteins
Capsid
Research Personnel
Viruses
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Bywaters, S. M., Brendle, S. A., Biryukov, J., Wang, J. W., Walston, J., Milici, J., ... Christensen, N. (2018). Production and characterization of a novel HPV anti-L2 monoclonal antibody panel. Virology, 524, 106-113. https://doi.org/10.1016/j.virol.2018.08.017
Bywaters, S. M. ; Brendle, S. A. ; Biryukov, J. ; Wang, J. W. ; Walston, J. ; Milici, J. ; Roden, R. B. ; Meyers, Craig ; Christensen, Neil. / Production and characterization of a novel HPV anti-L2 monoclonal antibody panel. In: Virology. 2018 ; Vol. 524. pp. 106-113.
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Bywaters, SM, Brendle, SA, Biryukov, J, Wang, JW, Walston, J, Milici, J, Roden, RB, Meyers, C & Christensen, N 2018, 'Production and characterization of a novel HPV anti-L2 monoclonal antibody panel', Virology, vol. 524, pp. 106-113. https://doi.org/10.1016/j.virol.2018.08.017

Production and characterization of a novel HPV anti-L2 monoclonal antibody panel. / Bywaters, S. M.; Brendle, S. A.; Biryukov, J.; Wang, J. W.; Walston, J.; Milici, J.; Roden, R. B.; Meyers, Craig; Christensen, Neil.

In: Virology, Vol. 524, 01.11.2018, p. 106-113.

Research output: Contribution to journalArticle

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Bywaters SM, Brendle SA, Biryukov J, Wang JW, Walston J, Milici J et al. Production and characterization of a novel HPV anti-L2 monoclonal antibody panel. Virology. 2018 Nov 1;524:106-113. https://doi.org/10.1016/j.virol.2018.08.017