Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10

R. Gandhimathi, G. Seghal Kiran, T. A. Hema, Joseph Selvin, T. Rajeetha Raviji, S. Shanmughapriya

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A sponge-associated marine actinomycetes Nocardiopsis alba MSA10 was screened and evaluated for the production of biosurfactant. Biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, lipase production and emulsification index. The active compound was extracted with three solvents including ethyl acetate, diethyl ether and dichloromethane. The diethyl ether extract was fractionated by TLC and semi-preparative HPLC to isolate the pure compound. In TLC, a single discrete spot was obtained with the R f 0.60 and it was extrapolated as valine. Based on the chemical characterization, the active compound was partially confirmed as lipopeptide. The optimum production was attained at pH 7, temperature 30°C, and 1% salinity with glucose and peptone supplementation as carbon and nitrogen sources, respectively. Considering the biosurfactant production potential of N. alba, the strain could be developed for large-scale production of lipopeptide biosurfactant.

Original languageEnglish (US)
Pages (from-to)825-835
Number of pages11
JournalBioprocess and biosystems engineering
Volume32
Issue number6
DOIs
StatePublished - Oct 1 2009

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Lipopeptides
Actinobacteria
Porifera
Ether
Peptones
Methylene Chloride
Salinity
Valine
Lipase
Oils
Nitrogen
Carbon
High Pressure Liquid Chromatography
Glucose
Temperature
Ethers
Emulsification
Dichloromethane
Lipases
Screening

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

Gandhimathi, R. ; Seghal Kiran, G. ; Hema, T. A. ; Selvin, Joseph ; Rajeetha Raviji, T. ; Shanmughapriya, S. / Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. In: Bioprocess and biosystems engineering. 2009 ; Vol. 32, No. 6. pp. 825-835.
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Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10. / Gandhimathi, R.; Seghal Kiran, G.; Hema, T. A.; Selvin, Joseph; Rajeetha Raviji, T.; Shanmughapriya, S.

In: Bioprocess and biosystems engineering, Vol. 32, No. 6, 01.10.2009, p. 825-835.

Research output: Contribution to journalArticle

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AU - Rajeetha Raviji, T.

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