Production of acetol from glycerol using engineered Escherichia coli

Hongliang Zhu, Xianyang Yi, Yi Liu, Hongbo Hu, Thomas Keith Wood, Xuehong Zhang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Escherichia coli Lin43 is a strain which has some mutations in glycerol kinase (GlpK) and the repressor for the glycerol 3-phosphate regulon (GlpR). When exposed to glycerol, it quickly accumulates lethal levels of methylglyoxal, which is a precursor of acetol; acetol is important for the manufacture of polyols, acrolein, dyes, and skin tanning agents. This work reports the engineering of E. coli Lin 43 for the conversion of glycerol into acetol. First, the glyoxalase system was interrupted by deleting the gloA gene, which increased the acetol yield by 32%. In addition, the aldehyde reductase YqhD was overexpressed which led to an increase of acetol production by 11.4-fold. Acetol production was optimized by varying the cell density, glycerol concentration, supplemental carbon source, pH and temperature. Under the optimal conditions (OD600=20, 20g/L glycerol, 2g/L succinate, pH 7.0, and 28°C), we obtained 5.4g/L acetol in 21h.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalBioresource technology
Volume149
DOIs
StatePublished - Jan 1 2013

Fingerprint

aldehyde
Glycerol
Escherichia coli
mutation
skin
dye
phosphate
fold
engineering
gene
carbon
temperature
Tanning
Glycerol Kinase
Pyruvaldehyde
Acrolein
Polyols
Aldehyde Reductase
Aldehydes
Succinic Acid

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Zhu, Hongliang ; Yi, Xianyang ; Liu, Yi ; Hu, Hongbo ; Wood, Thomas Keith ; Zhang, Xuehong. / Production of acetol from glycerol using engineered Escherichia coli. In: Bioresource technology. 2013 ; Vol. 149. pp. 238-243.
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Production of acetol from glycerol using engineered Escherichia coli. / Zhu, Hongliang; Yi, Xianyang; Liu, Yi; Hu, Hongbo; Wood, Thomas Keith; Zhang, Xuehong.

In: Bioresource technology, Vol. 149, 01.01.2013, p. 238-243.

Research output: Contribution to journalArticle

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