Production of antibodies to bovine adrenal tyrosine hydroxylase: cross reactivity studies with other pterin dependent hydroxylases

Thomas Lloyd, S. Kaufman

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A specific antiserum to purified bovine adrenal tyrosine hydroxylase was produced in sheep. Enzyme inhibition studies and agar double immunodiffusion reactions revealed extensive cross reactivity between the antiserum and tyrosine hydroxylases from bovine, rat, monkey, and rabbit adrenal glands, as well as from bovine caudate and human pheochromocytoma. The specificity of the antiserum was demonstrated by the lack of inhibition of phenylalanine hydroxylase, tryptophan hydroxylase, dopamine β hydroxylase, and tyrosinase.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalMolecular pharmacology
Volume9
Issue number4
StatePublished - Dec 1 1973

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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