TY - JOUR
T1 - Production of antibodies to bovine adrenal tyrosine hydroxylase
T2 - cross reactivity studies with other pterin dependent hydroxylases
AU - Lloyd, Thomas
AU - Kaufman, S.
PY - 1973/12/1
Y1 - 1973/12/1
N2 - A specific antiserum to purified bovine adrenal tyrosine hydroxylase was produced in sheep. Enzyme inhibition studies and agar double immunodiffusion reactions revealed extensive cross reactivity between the antiserum and tyrosine hydroxylases from bovine, rat, monkey, and rabbit adrenal glands, as well as from bovine caudate and human pheochromocytoma. The specificity of the antiserum was demonstrated by the lack of inhibition of phenylalanine hydroxylase, tryptophan hydroxylase, dopamine β hydroxylase, and tyrosinase.
AB - A specific antiserum to purified bovine adrenal tyrosine hydroxylase was produced in sheep. Enzyme inhibition studies and agar double immunodiffusion reactions revealed extensive cross reactivity between the antiserum and tyrosine hydroxylases from bovine, rat, monkey, and rabbit adrenal glands, as well as from bovine caudate and human pheochromocytoma. The specificity of the antiserum was demonstrated by the lack of inhibition of phenylalanine hydroxylase, tryptophan hydroxylase, dopamine β hydroxylase, and tyrosinase.
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M3 - Article
C2 - 4146888
AN - SCOPUS:0015798573
SN - 0026-895X
VL - 9
SP - 438
EP - 444
JO - Molecular Pharmacology
JF - Molecular Pharmacology
IS - 4
ER -