Production of antibodies to bovine adrenal tyrosine hydroxylase: cross reactivity studies with other pterin dependent hydroxylases

Thomas Lloyd, S. Kaufman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A specific antiserum to purified bovine adrenal tyrosine hydroxylase was produced in sheep. Enzyme inhibition studies and agar double immunodiffusion reactions revealed extensive cross reactivity between the antiserum and tyrosine hydroxylases from bovine, rat, monkey, and rabbit adrenal glands, as well as from bovine caudate and human pheochromocytoma. The specificity of the antiserum was demonstrated by the lack of inhibition of phenylalanine hydroxylase, tryptophan hydroxylase, dopamine β hydroxylase, and tyrosinase.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalMolecular pharmacology
Volume9
Issue number4
StatePublished - Dec 1 1973

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Pterins
Tyrosine 3-Monooxygenase
Mixed Function Oxygenases
Antibody Formation
Immune Sera
Phenylalanine Hydroxylase
Tryptophan Hydroxylase
Monophenol Monooxygenase
Immunodiffusion
Pheochromocytoma
Adrenal Glands
Agar
Haplorhini
Dopamine
Sheep
Rabbits
Enzymes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

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Production of antibodies to bovine adrenal tyrosine hydroxylase : cross reactivity studies with other pterin dependent hydroxylases. / Lloyd, Thomas; Kaufman, S.

In: Molecular pharmacology, Vol. 9, No. 4, 01.12.1973, p. 438-444.

Research output: Contribution to journalArticle

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