Production of anti‐H‐2 antibodies in thymectomized mice

Jan Klein, S. Livnat, V. Hauptfeld, L. Jeřábek, I. Weissman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Neonatally and adult thymectomized (Tx) mice of strains C57BL/10Sn and A. BY (H‐2b) were immunized at the age of 5 – 8 weeks with lethally irradiated spleen and lymph node cells from adult B 10. A and A (H‐2a) donors, respectively. The titer of H‐2 hemagglutinins and cytotoxins was determined at different intervals after immunization. Both untreated antibodies and antibodies reduced with 2‐mercaptoethanol (2‐Me) were tested. The non‐Tx control mice responded with production of both 2‐Me‐sensitive and 2‐Me‐resistant H‐2 antibodies. In the Tx mice, only 2‐Me‐sensitive H‐2 antibodies were detected. Thus, the anti‐H‐2 IgM response appears to be thymus‐independent and the anti‐H‐2 IgG response thymus‐dependent.

Original languageEnglish (US)
Pages (from-to)41-44
Number of pages4
JournalEuropean Journal of Immunology
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1974

Fingerprint

Antibody Formation
Antibodies
Cytotoxins
Hemagglutinins
Inbred C57BL Mouse
Immunoglobulin M
Immunization
Spleen
Immunoglobulin G
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Klein, J., Livnat, S., Hauptfeld, V., Jeřábek, L., & Weissman, I. (1974). Production of anti‐H‐2 antibodies in thymectomized mice. European Journal of Immunology, 4(1), 41-44. https://doi.org/10.1002/eji.1830040111
Klein, Jan ; Livnat, S. ; Hauptfeld, V. ; Jeřábek, L. ; Weissman, I. / Production of anti‐H‐2 antibodies in thymectomized mice. In: European Journal of Immunology. 1974 ; Vol. 4, No. 1. pp. 41-44.
@article{e209df2e73ee4521a735f84f95d6417f,
title = "Production of anti‐H‐2 antibodies in thymectomized mice",
abstract = "Neonatally and adult thymectomized (Tx) mice of strains C57BL/10Sn and A. BY (H‐2b) were immunized at the age of 5 – 8 weeks with lethally irradiated spleen and lymph node cells from adult B 10. A and A (H‐2a) donors, respectively. The titer of H‐2 hemagglutinins and cytotoxins was determined at different intervals after immunization. Both untreated antibodies and antibodies reduced with 2‐mercaptoethanol (2‐Me) were tested. The non‐Tx control mice responded with production of both 2‐Me‐sensitive and 2‐Me‐resistant H‐2 antibodies. In the Tx mice, only 2‐Me‐sensitive H‐2 antibodies were detected. Thus, the anti‐H‐2 IgM response appears to be thymus‐independent and the anti‐H‐2 IgG response thymus‐dependent.",
author = "Jan Klein and S. Livnat and V. Hauptfeld and L. Jeř{\'a}bek and I. Weissman",
year = "1974",
month = "1",
day = "1",
doi = "10.1002/eji.1830040111",
language = "English (US)",
volume = "4",
pages = "41--44",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "1",

}

Klein, J, Livnat, S, Hauptfeld, V, Jeřábek, L & Weissman, I 1974, 'Production of anti‐H‐2 antibodies in thymectomized mice', European Journal of Immunology, vol. 4, no. 1, pp. 41-44. https://doi.org/10.1002/eji.1830040111

Production of anti‐H‐2 antibodies in thymectomized mice. / Klein, Jan; Livnat, S.; Hauptfeld, V.; Jeřábek, L.; Weissman, I.

In: European Journal of Immunology, Vol. 4, No. 1, 01.01.1974, p. 41-44.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Production of anti‐H‐2 antibodies in thymectomized mice

AU - Klein, Jan

AU - Livnat, S.

AU - Hauptfeld, V.

AU - Jeřábek, L.

AU - Weissman, I.

PY - 1974/1/1

Y1 - 1974/1/1

N2 - Neonatally and adult thymectomized (Tx) mice of strains C57BL/10Sn and A. BY (H‐2b) were immunized at the age of 5 – 8 weeks with lethally irradiated spleen and lymph node cells from adult B 10. A and A (H‐2a) donors, respectively. The titer of H‐2 hemagglutinins and cytotoxins was determined at different intervals after immunization. Both untreated antibodies and antibodies reduced with 2‐mercaptoethanol (2‐Me) were tested. The non‐Tx control mice responded with production of both 2‐Me‐sensitive and 2‐Me‐resistant H‐2 antibodies. In the Tx mice, only 2‐Me‐sensitive H‐2 antibodies were detected. Thus, the anti‐H‐2 IgM response appears to be thymus‐independent and the anti‐H‐2 IgG response thymus‐dependent.

AB - Neonatally and adult thymectomized (Tx) mice of strains C57BL/10Sn and A. BY (H‐2b) were immunized at the age of 5 – 8 weeks with lethally irradiated spleen and lymph node cells from adult B 10. A and A (H‐2a) donors, respectively. The titer of H‐2 hemagglutinins and cytotoxins was determined at different intervals after immunization. Both untreated antibodies and antibodies reduced with 2‐mercaptoethanol (2‐Me) were tested. The non‐Tx control mice responded with production of both 2‐Me‐sensitive and 2‐Me‐resistant H‐2 antibodies. In the Tx mice, only 2‐Me‐sensitive H‐2 antibodies were detected. Thus, the anti‐H‐2 IgM response appears to be thymus‐independent and the anti‐H‐2 IgG response thymus‐dependent.

UR - http://www.scopus.com/inward/record.url?scp=0015980522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015980522&partnerID=8YFLogxK

U2 - 10.1002/eji.1830040111

DO - 10.1002/eji.1830040111

M3 - Article

VL - 4

SP - 41

EP - 44

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -