Production of monoclonal antibodies against chicken Pop1 (BVES)

Justin Robert Diangelo, Trusha K. Vasavada, William Cain, Melinda K. Duncan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Pop1 (BVES) is a member of the Popeye gene family which was named for its high-level expression in the heart and other muscle lineages. However, these proteins have no sequence similarity to any other gene family and their function is unclear. Here we report the production of recombinant chicken Pop1/BVES protein and the generation of two Pop1/BVES specific monoclonal antibodies (MAbs) 3F11-D9-E8 and 1B3-G11-A8. These antibodies detect recombinant Pop1/BVES in ELISAs and endogenous chicken Pop1/BVES in chicken heart extracts by Western blotting. Further, both 3F11-D9-E8 and 1B3-G11-A8 detect Pop1/BVES specifically in the cardiomyocytes of 8-day-old embryonic chicken hearts by immunofluorescence. These MAbs will be useful in immunolocalization and immunoblotting experiments of different tissue types to determine the location and levels of Pop1/BVES expression throughout development, as well as further analysis of the biochemistry of this protein.

Original languageEnglish (US)
Pages (from-to)377-381
Number of pages5
JournalHybridoma and Hybridomics
Volume20
Issue number5-6
DOIs
StatePublished - Jan 1 2001

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Chickens
Monoclonal Antibodies
Proteins
Immunoblotting
Cardiac Myocytes
Biochemistry
Genes
Fluorescent Antibody Technique
Myocardium
Western Blotting
Enzyme-Linked Immunosorbent Assay
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Diangelo, Justin Robert ; Vasavada, Trusha K. ; Cain, William ; Duncan, Melinda K. / Production of monoclonal antibodies against chicken Pop1 (BVES). In: Hybridoma and Hybridomics. 2001 ; Vol. 20, No. 5-6. pp. 377-381.
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Production of monoclonal antibodies against chicken Pop1 (BVES). / Diangelo, Justin Robert; Vasavada, Trusha K.; Cain, William; Duncan, Melinda K.

In: Hybridoma and Hybridomics, Vol. 20, No. 5-6, 01.01.2001, p. 377-381.

Research output: Contribution to journalArticle

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