Programmed cell death in the lithium pilocarpine model: Evidence for NMDA receptor and ceramide-mediated mechanisms

Mohamad A. Mikati, Elias Rizk, Shirine El Dada, Michele Zeinieh, Rana Kurdi, Jimmy El Hokayem, Amal Rahmeh, Mohamad Kobeissi, Diana Azzam, Julnar Usta, Marwan El Sabban, Ghassan Dbaibo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Ceramide is known to induce programmed cell death (PCD) in neural and non-neural tissues and to increase after kainic acid (KA) status epilepticus (SE). Ceramide increases have been shown to depend on NMDA receptor activation in the KA model, but these changes have not been studied in the lithium pilocarpine (LiPC) model. Thus, the purpose of this study was to determine if hippocampal ceramide levels increase after LiPC induced SE and if NMDA receptor blockade prevents PCD and any such ceramide increases. We found that LiPC induced SE resulted in ceramide increases and DNA fragmentation in the hippocampus of adult, P21, and P7 rats. The administration of MK-801, the NMDA receptor antagonist, in adults, 15 min prior to pilocarpine, prevented ceramide increases, and DNA fragmentation.

Original languageEnglish (US)
Pages (from-to)513-519
Number of pages7
JournalBrain and Development
Volume30
Issue number8
DOIs
StatePublished - Sep 2008

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Developmental Neuroscience
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Programmed cell death in the lithium pilocarpine model: Evidence for NMDA receptor and ceramide-mediated mechanisms'. Together they form a unique fingerprint.

Cite this