Promoter strength properties of the complete sigma E regulon of Escherichia coli and Salmonella enterica

Vivek K. Mutalik, Gen Nonaka, Sarah E. Ades, Virgil A. Rhodius, Carol A. Gross

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The σE-directed envelope stress response maintains outer membrane homeostasis and is an important virulence determinant upon host infection in Escherichia coli and related bacteria. σE is activated by at least two distinct mechanisms: accumulation of outer membrane porin precursors and an increase in the alarmone ppGpp upon transition to stationary phase. Expression of the σE regulon is driven from a suite of approximately 60 σE-dependent promoters. Using green fluorescent protein fusions to each of these promoters, we dissected promoter contributions to the output of the regulon under a variety of in vivo conditions. We found that the σE promoters exhibit a large dynamic range, with a few strong and many weak promoters. Interestingly, the strongest promoters control either transcriptional regulators or functions related to porin homeostasis, the very functions conserved among E. coli and its close relatives. We found that (i) the strength of most promoters is significantly affected by the presence of the upstream (-35 to -65) region of the promoter, which encompasses the UP element, a binding site for the C-terminal domain of the α-subunit of RNA polymerase; (ii) ppGpp generally activates σE promoters, and (iii) σE promoters are responsive to changing σE holoenzyme levels under physiological conditions, reinforcing the idea that the σE regulon is extremely dynamic, enabling cellular adaptation to a constantly changing environment.

Original languageEnglish (US)
Pages (from-to)7279-7287
Number of pages9
JournalJournal of bacteriology
Volume191
Issue number23
DOIs
StatePublished - Dec 2009

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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